CD19 B细胞、滤泡树突状细胞（克隆EP169）

广州健仑生物科技有限公司

在正常的淋巴组织中，CD19位于生发中心细胞（B细胞和滤泡树突状细胞）、套区细胞和滤泡间散在的细胞，整体的染色方式类似于CD20和CD22.但是与后两者不同的是，CD19也表达于前B细胞，流式检测方法还显示CD19可以在浆细胞中表达。在肿瘤组织中，CD19阳性见于绝大多数的B细胞肿瘤，浆细胞淋巴瘤以及T细胞肿瘤阴性。Masir的研究表明，CD19在14%弥漫性大B细胞淋巴瘤、30%的T细胞丰富的B细胞淋巴瘤和75%的移植后B淋巴增殖性疾病中阴性，在经典霍奇金病中的R-S细胞亦不表达。

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【产品介绍】

细胞定位：细胞膜

克隆号：MRQ-36

同型：IgG

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

CD19 B细胞、滤泡树突状细胞（克隆EP169）

（四）设计实验方法
实验方法的确定也应该根据实验目的进行。应该包括菌种的选择、配制何种培养基、需要哪些器材和药品、怎样接种、怎样培养、怎样观察结果等等?？晒┎慰嫉氖笛榉椒ㄈ缦拢?br />1、营养元素对黑曲霉生长的影响
⑴制备培养基：本实验分组配制培养基，培养基配方见附录1-8。培养基分装试管，每管装量4～5mL，0.1MPa灭菌30min后备用。
⑵孢子悬液制备：取无菌水1支，用接种环从黑曲霉斜面菌种管中挑取菌体2～3环，放入水中，充分混匀，备用。
⑶接种：每人取*、缺C、缺N、缺P、缺K和缺Zn培养液各一支，用1 mL无菌吸管按无菌操作法接种黑曲霉孢子悬液0.5 mL。本实验按每4人取一套培养基不接种作为对照。
⑷观察：接种后，将培养管置28℃温度下培养5～7d ,观察黑曲霉菌丝及孢子生长情况。
2、氧气对微生物生长的影响
⑴菌悬液制备：用接种环按无菌操作法取根瘤菌、巴斯德梭菌、大肠杆菌1～2环分别放入三支无菌水中制成菌悬液。
⑵接种：取已熔化并保温在50℃左右的培养基6支，用无菌吸管分别及取菌悬液0.2 mL接种到培养管中，每种试验菌接种2个重复，接种后立即置振荡器上混匀，并冷凝。
⑶培养：置培养管于28～30℃下培养3d。
⑷结果检查：取出培养管，观察并记录每支培养管的上、中、下各层次中细菌的菌苔大小，菌体集中分布的位置，以了解氧气对几种细菌生长的影响。
3、温度对微生物生长的影响
⑴接种：取抗原抗体蛋白胨琼脂斜面培养基8支，用接种环按无菌操作法分别在斜面上划线接种大肠杆菌与枯草杆菌，勿划破培养基。
⑵培养：将已接种的斜面培养基，分别放在4℃、28℃、37℃和45℃四种温度下培养。
⑶观察：培养48h、72h后观察生长状况，根据菌苔的大小确定其生长zui适温度。

CD19

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

(D) design experimental methods
The determination of experimental methods should also be based on experimental purposes. Should include the choice of bacteria, preparation of what kind of medium, what equipment and medicines are needed, how to inoculate, how to c*te, how to observe the results and so on. The experimental methods for reference are as follows:
1, nutrient elements on the growth of Aspergillus niger
⑴ Preparation of medium: The experiment group preparation medium, medium formula see Appendix 1-8. Medium sub-test tube, each tube loading 4 ~ 5mL, 0.1MPa sterilized 30min reserve.
⑵ spore suspension preparation: take sterile water 1, with the inoculation loop from Aspergillus niger bacteria tube picked 2 to 3 rings, into the water, mix well and set aside.
(3) Inoculation: Take a complete, absent C, absent N, absent P, deficient K and Zn-deficient culture medium, and inoculate 0.5 mL of Aspergillus niger spore suspension with sterile 1 mL sterile pipette. In this experiment, a set of culture medium was taken for every 4 persons as a control.
⑷ observation: After inoculation, the culture tube was incubated at 28 ° C for 5 to 7 days to observe the growth of Aspergillus niger and spores.
2, the impact of oxygen on microbial growth
⑴ bacterial suspension preparation: Inoculation loop according to aseptic method to take rhizobium, pasteurized bacteria, Escherichia coli 1 ~ 2 rings were placed in three sterile water to make bacterial suspension.
(2) Inoculation: take 6 mediums that have been melted and kept at about 50 ℃, inoculate them into the culture tube with sterile pipette and take 0.2 mL of the bacterial suspension respectively, and inoculate 2 replicates for each test bacteria and shake immediay after inoculation Mix well and condense.
⑶ culture: set culture tube at 28 ~ 30 ℃ for 3 days.
⑷ results check: remove the culture tube, observe and record each culture tube in the upper, middle and lower levels of bacteria in the lawn size, concentration of bacterial cells in the location to understand the impact of oxygen on the growth of several bacteria.
3, the impact of temperature on microbial growth
⑴ vaccination: take antigen antibody peptone agar slant medium 8, with a ring of vaccination according to aseptic technique respectively slant on the slant inoculated E. coli and Bacillus subtilis, do not scratch the medium.
⑵ culture: the slant culture medium has been inoculated, respectively, at 4 ℃, 28 ℃, 37 ℃ and 45 ℃ cultured at four temperatures.
⑶ observation: 48h, 72h after growth observed, according to the size of the lawn to determine the optimum temperature for growth.