国产日产欧美精品-亚洲国产综合久久精品-色综合色国产热无码一-亚洲欧美日本国产,免费观看一区二区三区_在线观看片A免费不卡观看_亚洲а∨天堂久久精品_99久无码中文字幕一本久道

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊

當(dāng)前位置:
美國布魯克海文儀器公司>技術(shù)文章>Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to c

技術(shù)文章

Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to c

閱讀:392          發(fā)布時間:2016-7-20

作者 Chia-Wei Lee, Lan-Ling Jang, Huei-Jyuan Pan, Yun-Ru Chen, Chih-Cheng Chen and Chau-Hwang Lee

 

 

摘要:Background

Cell membranes exhibit abundant types of responses to external stimulations. Intuitively, membrane topography should be sensitive to changes of physical or chemical factors in the microenvironment. We employed the non-interferometric wide-field optical profilometry (NIWOP) technique to quantify the membrane roughness of living neuroblastoma cells under various treatments that could change the mechanical properties of the cells.

 

Results

The membrane roughness was reduced as the neuroblastoma cell was treated with paclitaxel, which increases cellular stiffness by translocating microtubules toward the cell membranes. The treatment of positively charged gold nanoparticles (AuNPs) showed a similar effect. In contrast, the negatively charged AuNPs did not cause significant changes of the membrane roughness. We also checked the membrane roughness of fixed cells by using scanning electron microscopy (SEM) and confirmed that the membrane roughness could be regarded as a parameter reflecting cellular mechanical properties. Finally, we monitored the temporal variations of the membrane roughness under the treatment with a hypertonic solution (75 mM sucrose in the culture medium). The membrane roughness was increased within 1 h but returned to the original level after 2 h.

 

Conclusions

The results in the present study suggest that the optical measurement on membrane roughness can be regarded as a label-free method to monitor the changes in cell mechanical properties or binding properties of nanoparticles on cell surface. Because the cells were left untouched during the measurement, further tests about cell viability or drug efficacy can be done on the same specimen. Membrane roughness could thus provide a quick screening for new chemical or physical treatments on neuronal cells.

 

關(guān)鍵詞:Optical profilometry ;Neuroblastoma cell ;Paclitaxel ;Charged nanoparticle

 

 

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復(fù)您~

對比框

產(chǎn)品對比 產(chǎn)品對比 聯(lián)系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機(jī)商鋪
010-62081908
在線留言
龙口市| 时尚| 博白县| 兴义市| 隆回县| 始兴县| 安宁市| 炉霍县| 子长县| 敖汉旗| 泗阳县| 内丘县| 祁连县| 壤塘县| 东乡族自治县| 镶黄旗| 平利县| 凤台县| 海伦市| 交口县| 建湖县| 剑河县| 同仁县| 宿迁市| 武汉市| 宣恩县| 望城县| 濮阳县| 鄂尔多斯市| 阿拉善右旗| 梁河县| 两当县| 米林县| 手机| 龙游县| 蓬溪县| 房山区| 金沙县| 沧源| 龙游县| 宁强县|