国产日产欧美精品-亚洲国产综合久久精品-色综合色国产热无码一-亚洲欧美日本国产,免费观看一区二区三区_在线观看片A免费不卡观看_亚洲а∨天堂久久精品_99久无码中文字幕一本久道

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊

當(dāng)前位置:
美國布魯克海文儀器公司>技術(shù)文章>90Plus Zeta測量應(yīng)用案例-2016-15

技術(shù)文章

90Plus Zeta測量應(yīng)用案例-2016-15

閱讀:339          發(fā)布時間:2016-5-26

文獻(xiàn)名: A study of the synergistic effect of folate-decorated polymeric micelles incorporating Hydroxycamptothecin with radiotherapy on xenografted human cervical carcinoma

作者: Hong Youa, ShaoZhi Fua, XingHu Qinb, YanXin Yua, Bo Yanga, GuangPeng Zhanga, XiaoYang Suna, Yue Fengc, Yue Chenc, JingBo Wua
a Department of Oncology, the First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
b Department of Neurosurgery, the First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China
c Department of Nuclear Medicine, the First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, China


摘要:In this study, Hydroxycamptothecin (HCPT)-loaded micelles were formed in water by the self-assembly of folate (FA)-decorated amphiphilic block copolymer, methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL), and achieved a hydrodynamic diameter about of 132 nm. HCPT release from the micelles exhibited no initial burst but showed a sustained release profile. The cytotoxicity and targeting ability of FA conjugated polymeric micelles was investigated by using methylthiazoletetrazolium (MTT) and fluorescence microscopy. We found that FA-conjugated micelles had superior cytotoxicity against HeLa cells compared to non-conjugated micelles, and that they exerted this effect by folate receptor (FR)-mediated endocytosis. In addition, HeLa cells were xenografted into nude mice and subjected to radiotherapy (RT) and/or HCPT-loaded micelle treatment. The antitumor efficacy was detected by analysis of tumor growth delay (TGD) and median survival time. Micro fluorine-18-deoxyglucose PET/computed tomography (18F-FDG PET/CT) was performed to assess early tumor response to HCPT-loaded micelles in combination with RT. Analysis of cell cycle redistribution, apoptosis and expression of histone H2AX phosphorylation (λ-H2AX) was used to evaluate the mechanism by which HCPT loaded micelles led to radiosensitization. Taken together, the results showed that HCPT-loaded FA decorated micelles efficiently sensitized xenografts in mice to RT, and induced G2/M phase arrest, apoptosis and expression of λ-H2AX.

關(guān)鍵詞:Radiotherapy; 10-Hydroxycamptothecin; Micelle; Folate; Targeted drug delivery; Synergistic effect; Mechanism
 

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復(fù)您~

對比框

產(chǎn)品對比 產(chǎn)品對比 聯(lián)系電話 二維碼 在線交流

掃一掃訪問手機商鋪
010-62081908
在線留言
雅江县| 云霄县| 临湘市| 杭州市| 大同市| 定远县| 太康县| 右玉县| 永顺县| 杭州市| 资源县| 固原市| 绥德县| 安仁县| 鹤岗市| 依安县| 改则县| 望江县| 辽阳县| 沙河市| 拉孜县| 楚雄市| 多伦县| 陕西省| 肃宁县| 许昌市| 洛川县| 慈溪市| 民和| 禄丰县| 枣阳市| 海口市| 张家港市| 平潭县| 汾西县| 隆昌县| 孟州市| 庄浪县| 淳安县| 佳木斯市| 拜城县|