国产日产欧美精品-亚洲国产综合久久精品-色综合色国产热无码一-亚洲欧美日本国产,免费观看一区二区三区_在线观看片A免费不卡观看_亚洲а∨天堂久久精品_99久无码中文字幕一本久道

 

作者： Jing Hana, b, Xuebing Lia,

a CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 8 Zhongguancun Beiertiao, Beijing 100190, China

b Graduate University, Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing 100049, China

 

摘要：This paper describes the facile synthesis and bioevaluations of a novel conjugated polymer having tetrasaccharide SLeX branch and chitosan backbone (SLeX–chitosan conjugate). The synthesis was achieved by a convenient chemoenzymatic approach using an aldehyde-functionalized N-acetylglucosamine to branch it onto chitosan amino groups followed by three enzymatic reactions to further append galactose, sialic acid, and fucose residue to the branch. Surface plasmon resonance (SPR) study of SLeX–chitosan conjugate revealed a high affinity binding with E-selectin (Kd = 920 nM) and a potent inhibitory effect on the binding of E-selectin with SLeX–BSA (IC50 = 240 μM). By using biocompatible chitosan as the scaffold for presenting SLeX ligands, as well as the concise route tailored for the conjugate syntheses, the present study provides a practical method to explore safe and efficient anti-inflammatory agents.

 

關(guān)鍵詞：Chitosan; Sialyl Lewis X; Conjugated polymer; E-selectin; Anti-inflammatory agent