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美國布魯克海文儀器公司>技術(shù)文章>Hydrophobically modified polyethylenimine-based ternary complexes for targeting brain tumor: stabili

技術(shù)文章

Hydrophobically modified polyethylenimine-based ternary complexes for targeting brain tumor: stabili

閱讀:260          發(fā)布時(shí)間:2017-5-24
 作者 Brahmanand Dube, Abhijeet Pandey, Ganesh Joshi & Krutika Sawant

 

摘要:Hydrophobic modification of low molecular weight polyethylenimine (PEI 2kDa) by cholic acid (ChA) was done to obtain PEI2-ChA. The nanoplexes of PEI2-ChA with gWIZ-GFP demonstrated increase transfection efficiency (27%) in NT8e cell lines. The cell-cycle analysis of NT8e cells (p53 mutant) treated with transferrin containing nanoplexes showed increased apoptosis of cells. In vitro protein expression revealed expression of exogenous p53 protein. In vivo imaging of mice showed localized signal for GFP protein in brain region. The tumors of mice treated with transferrin containing nanoplexes of PEI2-ChA were 5 times smaller in size than the tumor of untreated animals.

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