Overview

MethoCult™ H4034 Optimum (MethoCult™ GF H4034) is a methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. MethoCult™ H4034 Optimum has been formulated to support optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). The inclusion of G-CSF in Optimum formulations improves discrimination of myeloid colony subtypes. This formulation is compatible with STEMvision™ software for automated colony counting.

Contains:

• Methylcellulose in Iscove's MDM 
• Fetal bovine serum 
• Bovine serum albumin 
• 2-Mercaptoethanol 
• Recombinant human stem cell factor (SCF) 
• Recombinant human interleukin 3 (IL-3) 
• Recombinant human erythropoietin (EPO) 
• Recombinant human granulocyte colony-stimulating factor (G-CSF) 
• Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) 
• Supplements

Subtype:

半固体培养基; 专业培养基

Cell Type:

造血干细胞和祖细胞

Species:

人; 非人灵长类动物

Application:

细胞培养; 集落检测; 功能检测

Brand:

MethoCult

Area of Interest:

药物发现和毒性测试; 干细胞生物学