Canine lysozyme(LYS)ELISA Kit
犬溶菌酶(LYS)ELISA試劑盒實(shí)驗(yàn)原理：
本試劑盒僅供研究使用，用于測(cè)定犬血清，血漿及相關(guān)液體樣本中溶菌酶（LYS）含量。應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中犬溶菌酶（LYS）水平。用純化的犬溶菌酶（LYS）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入溶菌酶（LYS），再與HRP標(biāo)記的LYS抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的溶菌酶（LYS）呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中犬溶菌酶（LYS）濃度。
Principle of the assay:
This kit allows for the determination of LYS concentrations in canine serum, blood plasma, and other biological fluids.Assay canine LYS level in the sample，use Purified canine LYS antibody to coat microtiter plate wells, make solid-phase antibody, then add LYS to wells, Combined LYS antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LYS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司