Complex of CDK7 (cyclin dependent kinase 7), Cyclin H1 and MNAT (MNAT1 component of CDK activating kinase).

Recombinant human CDK7, full length encompassing amino acids 2-346 (end), contains an N-terminal His-tag (6xHis). Recombinant human Cyclin H, full length encompassing amino acids 1-323 (end), contains a C-terminal His-tag (6xHis). Recombinant human MNAT, full length encompassing amino acids 2-309 (end), contains an N-terminal His-tag (6xHis). The three proteins were co-expressed in Sf9 insect cells and spontaneously formed a complex, which was affinity purified.