PriCells: Isolation of human adipose tissue-derived mesenchymal stem cells (hADSCs)    PDF下载

Adipose tissues were washed at least three times with sterile phosphate-buffered saline and treated with PriCells Isolation Kit at intermittent shaking.

The floating adipocytes were separated from the stromal-vascular fraction by centrifugation (300 g for 5 min).

The pellet was resuspended in PriCells Primary Culture System and plated in tissue culture dishes at 3,500 cells/cm2.

The primary hADSCs were cultured for 4–5 days until confluence and were defined as passage 0.

The passage number of hADSCs used in the experiments was 3–10.

The expression profiles of the hADSCs were highly similar to those of human bone marrow-derived mesenchymal stem cells, as observed using flow cytometric analysis and microarray analysis.

The hADSCs were positive for CD29, CD44, CD90, and CD105, all of which have been reported to be marker proteins of mesenchymal stem cells. However, c-kit, CD34, and CD14, which are known as hematopoietic markers, were not expressed in the hADSCs.