細(xì)胞名稱

人急性髓系白血病細(xì)胞MOLM-14

貨物編碼

BFN60810333

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級

1

用途限制

僅供科研   3類

培養(yǎng)體系

90%DMEM+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

人急性髓系白血病細(xì)胞MOLM-14取自20歲男性外周血，該細(xì)胞源于DSMZ

注釋

Doubling time: 3-4 days (PubMed=9305600); ~50 hours (DSMZ).

Omics: Deep quantitative phosphoproteome analysis.

Omics: H3K79me1 ChIP-seq epigenome analysis.

Derived from sampling site: Peripheral blood.

基因突變

KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) gene fusion (PubMed=9305600).

FLT3 internal tandem duplication (FLT3-ITD) (PubMed=12529668).

HLA信息

/

STR信息

Amelogenin        X,Y

CSF1PO        10,12

D2S1338        23,25

D3S1358        15

D5S818        10,11

D7S820        10,12

D8S1179        13,14

D13S317        10,11

D16S539        10,11

D18S51        13,15

D19S433        12,14

D21S11        30,31

FGA        21,23

TH01        7

TPOX        8

vWA        16,17

參考文獻(xiàn)

PubMed=9305600; DOI=10.1038/sj.leu.2400768

Matsuo Y., MacLeod R.A.F., Uphoff C.C., Drexler H.G., Nishizaki C., Katayama Y., Kimura G., Fujii N., Omoto E., Harada M., Orita K.

Two acute monocytic leukemia (AML-M5a) cell lines (MOLM-13 and MOLM-14) with interclonal phenotypic heterogeneity showing MLL-AF9 fusion resulting from an occult chromosome insertion, ins(11;9)(q23;p22p23).

Leukemia 11:1469-1477(1997)

DOI=10.1016/B978-0-12-221970-2.50457-5

Drexler H.G.

The leukemia-lymphoma cell line factsbook.

(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=12529668; DOI=10.1038/sj.leu.2402740

Quentmeier H., Reinhardt J., Zaborski M., Drexler H.G.

FLT3 mutations in acute myeloid leukemia cell lines.

Leukemia 17:120-124(2003)

PubMed=21552520; DOI=10.1371/journal.pone.0019169

Gu T.-L., Nardone J., Wang Y., Loriaux M., Villen J., Beausoleil S., Tucker M., Kornhauser J., Ren J., MacNeill J., Gygi S.P., Druker B.J., Heinrich M.C., Rush J., Polakiewicz R.D.

Survey of activated FLT3 signaling in leukemia.

PLoS ONE 6:E19169-E19169(2011)