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供貨周期	現(xiàn)貨	規(guī)格	T25培養(yǎng)瓶x1 1.5ml凍存管x2
貨號(hào)	BFN60808633	應(yīng)用領(lǐng)域	醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)
主要用途	僅供科研

細(xì)胞名稱(chēng)	人黑色素瘤細(xì)胞WM35
貨物編碼	BFN60808633
產(chǎn)品規(guī)格	T25培養(yǎng)瓶x1	1.5ml凍存管x2
細(xì)胞數(shù)量	1x10^6	1x10^6
保存溫度	37℃	-198℃
運(yùn)輸方式	常溫保溫運(yùn)輸	干冰運(yùn)輸
安全等級(jí)	1
用途限制	僅供科研 2類(lèi)

培養(yǎng)體系	DMEM高糖+10%FBS+1%三抗
培養(yǎng)溫度	37℃	二氧化碳濃度	5%
簡(jiǎn)介	人黑色素瘤細(xì)胞WM35取自24歲女性供體
注釋	Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Part of: Wistar Institute melanoma cell line collection. Microsatellite instability: Stable (MSS) (Sanger). Omics: Deep exome analysis. Omics: DNA methylation analysis. Omics: Transcriptome analysis. Caution: The reported STR profile from Wistar of this cell line was changed at one point between February 2016 when we retrieved them and entered them in the Cellosaurus and May 2018. The major changes were: D18S51: 20->14,15 and FGA: 19->19,24.
基因突變	Heterozygous for BRAF p.Val600Glu (c.1799T>A) (PubMed=18632627; Wistar).
HLA信息	/
STR信息	Amelogenin X CSF1PO 10 D2S1338 18,25 D3S1358 16,18 D5S818 11,12 D7S820 10,13 D8S1179 11,13 D13S317 11 D16S539 12,13 D18S51 14,15 D19S433 15,16 D21S11 29,32.2 FGA 19,24 TH01 9.3 TPOX 8 vWA 17
參考文獻(xiàn)	PubMed=23039341; DOI=10.1186/1476-4598-11-75 Byron S.A., Loch D.C., Wellens C.L., Wortmann A., Wu J., Wang J., Nomoto K., Pollock P.M. Sensitivity to the MEK inhibitor E6201 in melanoma cells is associated with mutant BRAF and wildtype PTEN status. Mol. Cancer 11:75-75(2012) PubMed=27087056; DOI=10.1038ep24569 Haridas P., McGovern J.A., Kashyap A.S., McElwain D.L.S., Simpson M.J. Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line. Sci. Rep. 6:24569-24569(2016) PubMed=27397505; DOI=10.1016/j.cell.2016.06.017 Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J. A landscape of pharmacogenomic interactions in cancer. Cell 166:740-754(2016) PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747 Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A. An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines. Cancer Res. 79:1263-1273(2019)

青旗（上海）生物技術(shù)發(fā)展有限公司，總部位于上海浦東新區(qū)，依托本地高校資源，逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺(tái)，在標(biāo)準(zhǔn)化細(xì)胞庫(kù)建立及細(xì)胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營(yíng)原代細(xì)胞、細(xì)胞系、ELISA試劑盒、感受態(tài)細(xì)胞和HPLC檢測(cè)等科研產(chǎn)品與服務(wù)。我們秉承對(duì)用戶負(fù)責(zé)的態(tài)度，以對(duì)科研的高度嚴(yán)謹(jǐn)，以嚴(yán)格的質(zhì)量控制，為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)！