細胞名稱

人肝癌細胞BEL-7402                  

貨物編碼

BFN60800695

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研用途               2類

培養(yǎng)體系

DMEM高糖培養(yǎng)基（Hyclone）+10%胎牛血清（Gibco）+1%雙抗（Hyclone）

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

人肝癌細胞BEL-7402細胞株是1974年從臨床肝癌手術(shù)標(biāo)本中建立的。細胞群體倍增時間為20小時。移植到處理過的wistar大鼠中的能形成腫瘤結(jié)節(jié)。細胞形態(tài)呈上皮樣細胞。在電子顯微鏡下亦顯示上皮細胞所具有的橋粒和張力原纖維，與臨床肝癌細胞相似。分瓶培養(yǎng)第四天時，其有絲分裂指數(shù)約為7%。BEL-7402細胞的染色體數(shù)為不足三倍體，有一個異常的近端著絲點染色體。間接免疫熒光法測BEL-7402細胞內(nèi)的AFP為陽性反應(yīng)。LDH同工酶譜顯示與成年人肝細胞不同，而與人胚肝及臨床肝癌相近。 

注釋

Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=28807831). Originally thought to originate from a 53 year old male patient hepatocellular carcinoma.

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00549.

Doubling time: 20 hours (PubMed=6154311); 31.2 +- 1.1 hours (PubMed=23167424).

Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).

Omics: Deep quantitative proteome analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

STR信息

Amelogenin:X；CSF1PO:10；D13S317:13.3；D16S539:9,10；D18S51:16；D19S433:13；D21S11:27,28；D2S1338:17；D3S1358:15,18；D5S818:12；D7S820:12；D8S1179:12；FGA:18,21；TH01:7；TPOX:12；vWA:16,18

參考文獻

PubMed=6154311; DOI=10.1360/ya1980-23-2-236

Chen R.-M., Zhu D.-H., Ye X.-Z., Shen D.-W., Lu R.-H.

Establishment of three human liver carcinoma cell lines and some of their biological characteristics in vitro.

Sci. Sin. 23:236-247(1980)

PubMed=6275024; DOI=10.1099/0022-1317-57-1-95

Koshy R., Maupas P., Muller R., Hofschneider P.H.

Detection of hepatitis B virus-specific DNA in the genomes of human hepatocellular carcinoma and liver cirrhosis tissues.

J. Gen. Virol. 57:95-102(1981)

DOI=10.1007/978-4-431-68349-0_4

Alexander J.J.

Human hepatoma cell lines.

(In) Neoplasms of the liver; Okuda K., Ishak K.G. (eds.); pp.47-56; Springer; Tokyo (1987)

PubMed=1849045

Fuhrer J.P., Xie H., Murphy M.J. Jr., Ye J.N., Yao Z.

Characterization of a membrane-associated glycoprotein (gp 43) on human hepatocellular carcinoma by a monoclonal antibody.

Cancer Res. 51:2158-2163(1991)

PubMed=14571772

Liu L.-X., Jiang H.-C., Liu Z.-H., Zhu A.-L., Zhou J., Zhang W.-H., Wang X.-Q., Wu M.

Gene expression profiles of hepatoma cell line BEL-7402.

Hepato-Gastroenterol. 50:1496-1501(2003)

PubMed=23167424; DOI=10.7314/APJCP.2012.13.9.4807

Gu W., Fang F.-F., Li B., Cheng B.-B., Ling C.-Q.

Characterization and resistance mechanisms of a 5-fluorouracil-resistant hepatocellular carcinoma cell line.

Asian Pac. J. Cancer Prev. 13:4807-4814(2012)

PubMed=23505090; DOI=10.1002/hep.26402

Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.

Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.

Hepatology 58:706-717(2013)

PubMed=28807831; DOI=10.1016/j.jhep.2017.08.002

Rebouissou S., Zucman-Rossi J., Moreau R., Qiu Z.-X., Hui L.-J.

Note of caution: contaminations of hepatocellular cell lines.

J. Hepatol. 67:896-897(2017)