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供貨周期	現(xiàn)貨	規(guī)格	T25培養(yǎng)瓶x1 1.5ml凍存管x2
貨號(hào)	BFN60810358	應(yīng)用領(lǐng)域	醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)
主要用途	僅供科研

青旗（上海）生物技術(shù)發(fā)展有限公司，總部位于上海浦東新區(qū)，依托本地高校資源，逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺(tái)，在標(biāo)準(zhǔn)化細(xì)胞庫建立及細(xì)胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營(yíng)原代細(xì)胞、細(xì)胞系、ELISA試劑盒、感受態(tài)細(xì)胞和HPLC檢測(cè)等科研產(chǎn)品與服務(wù)。我們秉承對(duì)用戶負(fù)責(zé)的態(tài)度，以對(duì)科研的高度嚴(yán)謹(jǐn)，以嚴(yán)格的質(zhì)量控制，為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)！

詳情介紹

細(xì)胞名稱	人B淋巴細(xì)胞GM12878
貨物編碼	BFN60810358
產(chǎn)品規(guī)格	T25培養(yǎng)瓶x1	1.5ml凍存管x2
細(xì)胞數(shù)量	1x10^6	1x10^6
保存溫度	37℃	-198℃
運(yùn)輸方式	常溫保溫運(yùn)輸	干冰運(yùn)輸
安全等級(jí)	1
用途限制	僅供科研 3類

培養(yǎng)體系	90%DMEM(不含丙酮酸鈉）+10%FBS+1%三抗
培養(yǎng)溫度	37℃	二氧化碳濃度	5%
簡(jiǎn)介	人B淋巴細(xì)胞GM12878取自女性外周血。
注釋	Part of: CEPH/Utah pedigree cell line collection. Part of: ENCODE project common cell types; tier 1. Part of: Genetic Testing Reference Material (GeT-RM) samples. Part of: Genome in a Bottle (GIAB) consortium samples. Part of: International Genome Sample Resource (1000 genomes project) cell lines. Registration: CEPH Families Reference Panel; 146302. Registration: National Institute of Standards and Technology, Standard Reference Materials; HG001. Population: Utah residents with ancestry from Northern and Western Europe. Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). Omics: CNV analysis. Omics: Deep exome analysis. Omics: Deep proteome analysis. Omics: DNA methylation analysis. Omics: Deep RNAseq analysis. Omics: Genome sequenced. Omics: H3K27ac ChIP-seq epigenome analysis. Omics: H3K27me3 ChIP-seq epigenome analysis. Omics: H3K36me3 ChIP-seq epigenome analysis. Omics: H3K4me1 ChIP-seq epigenome analysis. Omics: H3K4me2 ChIP-seq epigenome analysis. Omics: H3K4me3 ChIP-seq epigenome analysis. Omics: H3K79me2 ChIP-seq epigenome analysis. Omics: H3K9ac ChIP-seq epigenome analysis. Omics: H3K9me3 ChIP-seq epigenome analysis. Omics: H4K20me1 ChIP-seq epigenome analysis. Omics: Mitochondrial genome sequenced. Omics: SNP array analysis. Omics: Transcriptome analysis. Derived from sampling site: Peripheral blood.
基因突變	*CYP2C19 p.Pro227Pro (c.681G>A); cryptic splice acceptor activation; allele CYP2C192 (Coriell).**
HLA信息	/
STR信息	/
參考文獻(xiàn)	PubMed=21418647; DOI=10.1186/gb-2011-12-3-r25 Morcos L., Ge B., Koka V., Lam K.C., Pokholok D.K., Gunderson K.L., Montpetit A., Verlaan D.J., Pastinen T. Genome-wide assessment of imprinted expression in human cells. Genome Biol. 12:R25.1-R25.14(2011) PubMed=23325432; DOI=10.1101/gr.147942.112 Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M. Dynamic DNA methylation across diverse human cell lines and tissues. Genome Res. 23:555-567(2013) PubMed=23676674; DOI=10.1038/nature12223 Wu L., Candille S.I., Choi Y., Xie D., Jiang L., Li-Pook-Than J., Tang H., Snyder M. Variation and genetic control of protein abundance in humans. Nature 499:79-82(2013) PubMed=24185094; DOI=10.1038/nbt.2728 Selvaraj S., Dixon J.R., Bansal V., Ren B. Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing. Nat. Biotechnol. 31:1111-1118(2013) PubMed=26621101; DOI=10.1016/j.jmoldx.2015.08.005 Pratt V.M., Everts R.E., Aggarwal P., Beyer B.N., Broeckel U., Epstein-Baak R., Hujsak P., Kornreich R., Liao J., Lorier R., Scott S.A., Smith C.H., Toji L.H., Turner A., Kalman L.V. Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: a GeT-RM collaborative project. J. Mol. Diagn. 18:109-123(2016) PubMed=30485824; DOI=10.1016/j.celrep.2018.10.096 Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R., Easwaran H., Baylin S.B., Slamon D., Velculescu V.E., Scharpf R.B. Integrated genomic, epigenomic, and expression analyses of ovarian cancer cell lines. Cell Rep. 25:2617-2633(2018) PubMed=31273215; DOI=10.1038/s41597-019-0116-4 Osorio D., Yu X., Yu P., Serpedin E., Cai J.J. Single-cell RNA sequencing of a European and an African lymphoblastoid cell line. Sci. Data 6:112-112(2019) PubMed=31401124; DOI=10.1016/j.jmoldx.2019.06.007 Gaedigk A., Turner A., Everts R.E., Scott S.A., Aggarwal P., Broeckel U., McMillin G.A., Melis R., Boone E.C., Pratt V.M., Kalman L.V. Characterization of reference materials for genetic testing of CYP2D6 alleles: a GeT-RM collaborative project. J. Mol. Diagn. 21:1034-1052(2019)