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清華大學(xué)免疫學(xué)醫(yī)學(xué)院轉(zhuǎn)染BMDMs細胞發(fā)表文章到JEM已見刊
閱讀：1376 發(fā)布時間：2020-6-15
一、2020一季度，清華大學(xué)免疫學(xué)與醫(yī)學(xué)院；清華大學(xué)生命科學(xué)學(xué)院聯(lián)合發(fā)表標題為：The colonic macrophage transcription factor RBP-J orchestrates intestinal immunity against bacterial pathogens的文章到JEM；
二、用invigentech（英克)INVI DNA RNA Transfection Reagent轉(zhuǎn)染試劑；
三、成功轉(zhuǎn)染BMDMs細胞(小鼠骨髓來源的巨噬細胞)發(fā)表文章見刊；
四、文章簡介：結(jié)腸巨噬細胞轉(zhuǎn)錄因子RBP-J協(xié)調(diào)腸道對細菌的免疫病原體研究.
五、文章部分內(nèi)容見下：
RNA-mediated interference
siRNA specifically targeting mouse Cebpb and nontargeting control siRNA were purchased from GenePharma Company. siRNA was transfected into BMDMs using INVI DNA RNA Transfection Reagent according to the manufacturer’s in structions (Invigentech). Cells were used 48 h after transfection.