BTTAA 是最新一代的 CuAAC 水溶性加速配体，与上一代配体（例如 THPTA 或 TBTA）相比，它提供了更大的速率增强。更重要的是，它最大限度地减少了对所探测细胞或生物体生理状态的扰动，并通过进一步降低催化剂配方中的铜负载量，实现了有效的生物偶联，抑制了细胞毒性。

BTTAA is a newest generation, water-soluble accelerating ligand for CuAAC that provides much greater rate enhancement compared to previous generation ligands (e.g. THPTA or TBTA). More importantly, it minimizes perturbations to the physiological state of the cells or organisms probed and allows for effective bioconjugation with suppressed cell cytotoxicity by further lowering copper loading in the catalyst formulation.