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Jackson|二抗111-035-045*
通过免疫亲和层析从抗血清分离全IgG抗体作为完整分子。
通过免疫亲和层析从抗血清分离全IgG抗体作为完整分子。
详细介绍
Jackson|二抗111-035-045*
通过免疫亲和层析从抗血清分离全IgG抗体作为完整分子。 它们具有Fc部分和两个通过二硫键连接在一起的抗原结合Fab部分，因此它们是二价的。 据报道平均分子量约为160kDa。 整个IgG形式的抗体适用于大多数免疫检测程序，并且是成本效益的。
基于免疫电泳和/或ELISA，抗体与全分子兔IgG反应。 它还与其他兔免疫球蛋白的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的抗体。 已通过ELISA和/或固相吸附测试抗体以确保与人血清蛋白的小交叉反应，但其可与来自其他物种的免疫球蛋白交叉反应。
Jackson|二抗111-035-045*
Physical State: Freeze-dried solid
Storage:
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Suggested Working Concentration or Dilution Range:
1:500 - 1:5,000 for immunohisto/cytochemistry
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.