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Jackson|二抗715-167-003*

通过木瓜蛋白酶消化完整IgG抗体产生Fab片段抗体，以去除整个Fc部分，包括铰链区。 这些抗体是单价的，仅含有单个抗原结合位点。 Fab片段的分子量为约50kDa。

基于抗原结合测定和/或ELISA，抗体与全分子小鼠IgG反应。 它还与其他小鼠免疫球蛋白的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的抗体。 抗体可以与来自其他物种的免疫球蛋白交叉反应。

Jackson|二抗715-167-003*

Physical State: Freeze-dried solid 
Storage:

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. 
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative: 0.05% Sodium Azide 

Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.