thermo二抗A21203|Invitrogen抗体*

Alexa Fluor染料是目前值得信赖的荧光染料之一。 Invitrogen™Alexa Fluor 594染料是一种明亮的红色荧光染料，具有理想的激发，适用于594 nm激光线。为了在成像和流式细胞仪中产生稳定的信号，Alexa Fluor 594染料在很宽的摩尔范围内对pH不敏感。具有高荧光量子产率和高光稳定性的探针允许以高灵敏度检测低丰度生物结构。 Alexa Fluor 594染料分子可以高摩尔比附着在蛋白质上而不会发生明显的自猝灭，从而实现更明亮的结合物和更灵敏的检测。每种缀合物的标记程度通常为每个IgG分子2-8个荧光团分子;每个产品批次的分析证书上都标明了确切的标签程度。

这些驴抗小鼠IgG（H + L）全二级抗体已经过亲和纯化，并显示出与牛，鸡，山羊，豚鼠，仓鼠，马，人，兔，大鼠和绵羊血清蛋白的小交叉反应性。交叉吸附或预吸附是提高抗体特异性的纯化步骤，导致更高的灵敏度和更少的背景染色。使第二抗体溶液通过含有来自潜在交叉反应性物质的固定化血清蛋白的柱基质。在柱中仅捕获非特异性结合的二抗，并且高度特异性的第二抗体流过。这种额外步骤的益处在多重/多色染色实验（例如，流式细胞术）中是显而易见的，其中存在与其他一抗的潜在交叉反应性或在可能存在内源免疫球蛋白的组织/细胞荧光染色实验中。

thermo二抗A21203|Invitrogen抗体*

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

4 µg/mL

Immunofluorescence (IF)

4 µg/mL

Immunohistochemistry (IHC)

1-10 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 5 publications below

Immunocytochemistry (ICC)

See 6 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 3 publications below

Miscellaneous PubMed (MISC)

See 14 publications below

Product Specifications

Species Reactivity

Mouse

Host / Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor® 594

Excitation/Emission Profile

View spectra 

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

RRID

AB_2535789

Target

IgG

Antibody Form

Whole Antibody

Target Information

We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.

Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.

Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.