Sci Total Environ:抑制鉀信號(hào)影響地桿菌生物膜的形成
??NMT作為生命科學(xué)底層核心技術(shù),是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年,NMT已扎根中國(guó)15年。2020年,中國(guó)NMT銷(xiāo)往瑞士蘇黎世大學(xué),正式打開(kāi)歐洲市場(chǎng)。
研究使用平臺(tái):NMT生物膜創(chuàng)新科研平臺(tái)
期刊:Science of the Total Environment
主題:抑制鉀信號(hào)影響地桿菌生物膜的形成
標(biāo)題:Potassium channel blocker inhibits the formation and electroactivity of Geobacter biofilm
影響因子:5.589
檢測(cè)指標(biāo):K+流速
檢測(cè)樣品:地桿菌
K+流實(shí)驗(yàn)處理方法:
地桿菌分別在0mM、5mM、10mM的TEA中處理
K+流實(shí)驗(yàn)測(cè)試液成份:
0.1mM KCl、1mM glucose
作者:福建農(nóng)林大學(xué)陳姍姍、靖憲月
中文摘要(谷歌機(jī)翻)
生物膜中的細(xì)菌能夠利用鉀離子通道介導(dǎo)的電信號(hào)來(lái)實(shí)現(xiàn)細(xì)胞間的通信。然而,目前尚不清楚當(dāng)被強(qiáng)電場(chǎng)包圍時(shí),這些信號(hào)是否在Geobacter sp。中起作用。
這項(xiàng)研究使用了鉀通道阻滯劑(四乙銨,TEA),該阻滯劑干擾K+的釋放,但不干擾細(xì)菌的生長(zhǎng),以證明鉀離子通道介導(dǎo)的電信號(hào)傳導(dǎo)影響了Geobacter sulfreducens的形成和電活性。結(jié)果表明,5 mM TEA減慢了地球還原硫細(xì)菌生物膜的形成,電流密度比對(duì)照組低約50%。
電化學(xué)分析表明,加入TEA的生物膜的電活性較差。特別是,具有TEA的微米級(jí)生物膜表現(xiàn)出較少的高電流峰,并且參與還原性減少硫桿菌的生物膜中電子轉(zhuǎn)移的外層基團(tuán)的物種與對(duì)照組不同。
這項(xiàng)工作提供了初步的證據(jù)來(lái)揭示鉀通道在還原性土壤桿菌中的電活性生物膜的作用。
Mean net K+ fluxmeasured from the biofilms in response to treatment with 5 mM
英文摘要
Bacteria in biofilms are able to utilize potassium ion channel mediated electrical signaling to achieve cell–cell communication. However, it remains unclear whether these signals play a role in Geobacter sp.when surrounded by an intense electric field.
This study used a potassium channel blocker (tetraethylammonium, TEA) that interfered with the release of K+ but not bacterial growth to demonstrate that potassium ion channel-mediated electrical signaling affected the formation and electroactivity of Geobacter sulfurreducens. The results showed that 5 mM TEA slowed the formation of Geobacter sulfurreducens biofilm, and the current density was ~50% lower than in the control.
The electrochemical analyses showed that the electroactivity of the biofilms with TEA addition was inferior. In particular, the micrometer- scale biofilm with TEA exhibited fewer high current peaks, and the species of outermost groups that participated in the electron transfer in Geobacter sulfurreducens biofilms was different from the control.
This work provides initial evidence to reveal the role of potassium channels in Geobacter sulfurreducens electroactive biofilms.