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NMT歷*的今天丨Ca2+流指示的微藻氮脅迫信號(hào)轉(zhuǎn)導(dǎo)研究
NMT是基因功能的活體檢測(cè)技術(shù)，已被103位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用。
NMT歷*的今天
2014年01月14日，中科院水生所王強(qiáng)、陳輝用NMT在Plant and Cell Physiology上發(fā)表了標(biāo)題為Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp. C2的研究成果。'
期刊：Plant and Cell Physiology
主題：Ca2+流指示的微藻氮脅迫信號(hào)轉(zhuǎn)導(dǎo)研究
標(biāo)題：Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp. C2
影響因子：4.134
檢測(cè)指標(biāo)：Ca2+流速
作者：中科院水生所王強(qiáng)、陳輝
英文摘要
Changes in the cytosolic Ca2+ levels and the role of Ca2+ signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca2+ influx across the plasma membrane into cells.
Ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this Ca2+ influx on the generation of the Ca2+ signal, and the results showed that the cytosolic Ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. However, the increase could be inhibited by pre-treatment with the Ca2+ channel blockers ruthenium red, verapamil and GdCl3, indicating that both the influx of Ca2+ from the extracellular space via Ca2+ channels that are localized in the plasma membrane and the release of Ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the Ca2+ signal.
During nitrogen starvation, neutral lipid synthesis in Chlorella sp. C2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three Ca2+ channel blockers, demonstrating the regulation of Ca2+ via these Ca2+ channels in neutral lipid synthesis.
The results suggested that by transduction of extracellular stress signals into the cell and the regulation of the Ca2+ signal in neutral lipid synthesis, Ca2+ signal transduction played important roles in the response mechanism of Chlorella sp. C2 to nitrogen starvation.
中文摘要（谷歌機(jī)翻）
小球藻中胞質(zhì)Ca2+水平的變化以及Ca2+信號(hào)轉(zhuǎn)導(dǎo)在中性脂質(zhì)合成中的作用。研究了氮饑餓條件下的C2。通過使用掃描離子選擇電極技術(shù)檢測(cè)到的結(jié)果表明，氮饑餓導(dǎo)致大量Ca2+穿過質(zhì)膜流入細(xì)胞。
Ca2+熒光成像和流式細(xì)胞儀用于評(píng)估這種Ca2+內(nèi)流對(duì)Ca2+信號(hào)生成的影響，結(jié)果表明，當(dāng)細(xì)胞暴露于細(xì)胞中時(shí)，胞漿中Ca2+的濃度會(huì)瞬時(shí)增加，然后保持穩(wěn)定的高水平。氮饑餓。但是，通過用Ca2+通道阻滯劑釕紅，維拉帕米和GdCl3進(jìn)行預(yù)處理可以抑制這種增加，這表明Ca2+經(jīng)由位于質(zhì)膜上的Ca2+通道從細(xì)胞外空間大量涌入以及Ca2+的釋放。Ca2+信號(hào)的產(chǎn)生和轉(zhuǎn)導(dǎo)需要通過內(nèi)部鈣儲(chǔ)存器進(jìn)行細(xì)胞內(nèi)鈣儲(chǔ)存。
在氮饑餓期間，小球藻中的中性脂質(zhì)合成。通過使用三種Ca2+通道阻滯劑的預(yù)處理，C2對(duì)應(yīng)激條件的響應(yīng)也受到了不同程度的抑制，這表明在中性脂質(zhì)合成中通過這些Ca2+通道對(duì)Ca2+的調(diào)節(jié)。
結(jié)果表明，通過將細(xì)胞外應(yīng)激信號(hào)轉(zhuǎn)導(dǎo)到細(xì)胞中以及中性脂質(zhì)合成中Ca2+信號(hào)的調(diào)節(jié)，Ca2+信號(hào)轉(zhuǎn)導(dǎo)在小球藻的反應(yīng)機(jī)制中發(fā)揮了重要作用。C2導(dǎo)致氮饑餓。
Fig. 1 The total Ca2+ ?ux rates over 5 min in Chlorella sp. C2 under N starvation. (a) Microphotographic examples of Ca2+ ion ?ux/voltage-clamp measurements. (b) Total ?ux rates of Ca2+ were detected at 0, 0.5, 2 and 8 d after N starvation. The columns represent the means of three replicated studies in each sample, with the SD of the means (t-test, P < 0.05). The signi?cance of the differences between the control (0 d) and other test values was tested using a one-way analysis of variance. *P < 0.05 vs. control.