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中國臺(tái)灣師大 | AgNPs和CuNPs抑制斑馬魚胚胎側(cè)線毛細(xì)胞鈣吸收
NMT是基因功能的活體檢測技術(shù)，已被103位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用。
期刊：Aquatic Toxicology
主題：AgNPs和CuNPs抑制斑馬魚胚胎側(cè)線毛細(xì)胞鈣吸收
標(biāo)題：Toxic e?ects of silver and copper nanoparticles on lateral-line hair cells of zebra?sh embryos
影響因子：3.794
檢測指標(biāo)：Ca2+流速
檢測樣品：斑馬魚胚胎側(cè)線毛細(xì)胞發(fā)束
Ca2+流實(shí)驗(yàn)處理方法：
斑馬魚胚胎經(jīng)0-96hpf受精后，分別用0，0.1，1，3ppm的AgNPs和0，0.01，0.1，1ppm的CuNPs進(jìn)行受精
Ca2+流實(shí)驗(yàn)測試液成份：
0.5mM NaCl，0.2mM CaSO4，0.2mM MgSO4，0.16mM KH2PO4，and0.16mM K2HPO4（pH 7.0），300μM MOPS，0.1mg/l tricaine
作者：中國臺(tái)灣師范大學(xué)林豊益
斑馬魚毛細(xì)胞離子流檢測
英文摘要
The potential toxicity of nanoparticles (NPs) to the early stages of fish is still unclear. In this study, we investigated the toxic effects of silver (AgNPs) and copper nanoparticles (CuNPs) on lateral-line hair cells of zebrafish embryos.
Zebrafish embryos were incubated in different concentrations of AgNPs and CuNPs at 0˜96 h post-fertilization (hpf). Both AgNPs and CuNPs were found to cause toxic effects in zebrafish embryos in a dosedependent manner. Values of the 96-h 50% lethal concentration (LC50) of AgNPs and CuNPs were 6.1 ppm (56.5 μM) and 2.61 ppm (41.1 μM), respectively. The number of FM1-43-labeled hair cells and the microstructure of hair bundles were significantly impaired by AgNPs [≥1 ppm (9.3 μM)] and CuNPs [≥0.01 ppm (0.16 μM)].
Ca2+ influxes at hair bundles of hair cells were measured with a scanning ion-selective microelectrode technique to evaluate the function of hair cells. AgNPs [≥0.1 ppm (0.9 μM)] and CuNPs [≥0.01 ppm (0.16 μM)] were both found to significantly reduce Ca2+ influxes. Similar toxic effects were also found in hatched embryos subjected to 4 h of exposure (96˜100 hpf) to AgNPs and CuNPs.
This study revealed that lateralline hair cells of zebrafish are susceptible to AgNPs and CuNPs, and these contaminants in aquatic environments could pose a threat to fish survival.
中文摘要（谷歌機(jī)翻）
納米粒子（NPs）對魚類早期的潛在毒性仍不清楚。在這項(xiàng)研究中，我們調(diào)查了銀（AgNPs）和銅納米顆粒（CuNPs）對斑馬魚胚胎側(cè)線毛細(xì)胞的毒性作用。
斑馬魚胚胎在受精后0?96 h（hpf）的不同濃度的AgNPs和CuNPs中孵育。發(fā)現(xiàn)AgNPs和CuNPs均以劑量依賴性方式引起斑馬魚胚胎的毒性作用。AgNP和CuNP的96小時(shí)50％致死濃度（LC50）值分別為6.1 ppm（56.5μM）和2.61 ppm（41.1μM）。AgNPs [≥1ppm（9.3μM）]和CuNPs [≥0.01ppm（0.16μM）]嚴(yán)重?fù)p害了FM1-43標(biāo)記的毛細(xì)胞數(shù)量和發(fā)束的微觀結(jié)構(gòu)。
用掃描離子選擇微電極技術(shù)測量在毛細(xì)胞的發(fā)束處的Ca 2+流入，以評估毛細(xì)胞的功能。發(fā)現(xiàn)AgNPs [≥0.1ppm（0.9μM）]和CuNPs [≥0.01ppm（0.16μM）]均能顯著減少Ca2 +的流入。在暴露于AgNPs和CuNPs 4小時(shí)（96?100 hpf）的孵化胚胎中也發(fā)現(xiàn)了類似的毒性作用。
這項(xiàng)研究表明，斑馬魚的側(cè)線毛細(xì)胞對AgNPs和CuNPs敏感，而這些污染物在水生環(huán)境中可能對魚類的生存構(gòu)成威脅。
結(jié)果表明：在AgNP處理組中，相比于0 ppm，Ca2+吸收速率在0.1,1和3 ppm時(shí)分別減少了26%,46%和91%;在CuNP處理組中，相比于0 ppm，Ca2+吸收速率在0.01 ppm時(shí)減少了38%，而在0.1和1 ppm時(shí)幾乎檢測不到Ca2+的吸收速率。由此可見，AgNPs （≥0.1 ppm）和 CuNPs（≥0.01ppm）都會(huì)顯著抑制Ca2+的吸收。海洋環(huán)境中的污染物會(huì)威脅魚類的生存。