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【公司名称】 广州健仑生物科技有限公司
【市场部】 欧
【】
【腾讯 】
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
组成蛋白质的氨基酸有20种,根据密码子摆动学说至少需要31种
tRNA,但在脊椎动物中只存在22种tRNA。1969年以来,研究了来
自各种不同生物:如酵母、大肠杆菌、小麦、鼠等十几种tRNA的
结构,证明它们的碱基序列都能折叠成三叶草形二级结构(图3-
23),而且都具有如下的共性:①5’末端具有G(大部分)或C。
②3’末端都以CCA的顺序终结。③有一个富有鸟嘌呤的环。④有
一个反密码子环,在这一环的顶端有三个暴露的碱基,称为反密
码子(anticodon).反密码子可以与mRNA链上互补的密码子配对
。⑤有一个胸腺嘧啶环。又称核糖体RNA(ribosomalRNA),
rRNA是组成核糖体的主要成分。核糖体是合成蛋白质的工厂。在
大肠杆菌中,rRNA量占细胞总RNA量的75%-85%,而tRNA占15%,
mRNA仅占3-5%。rRNA一般与核糖体蛋白质结合在一起,形成核糖
体(ribosome)。大肠杆菌核糖体的30S亚基由1分子沉降系数为
16S的rRNA和21个核糖体蛋白组成。50S亚基则由2个rRNA(23S
+5S)和34个核糖体蛋白组成。真核生物的核糖体更加复杂,由1
个以上的rRNA分子和更多的蛋白质组成。如果把rRNA从核糖体上
除掉,核糖体的结构就会发生塌陷。S为沉降系数
(sedimentationcoefficient),当用超速离心测定一个粒子
的沉淀速度时,此速度与粒子的大小直径成比例。5S含有120个核
苷酸,16S含有1540个核苷酸,而23S含有2900个核苷酸。而真核
生物有4种rRNA,它们分子大小分别是5S、5.8S、18S和28S,分
别具有大约120、160、1900和4700个核苷酸。rRNA是单链,它包
含不等量的A与U、G与C,但是有广泛的双链区域。在双链区,碱
基因氢键相连,表现为发夹式螺旋。rRNA在蛋白质合成中的功能
尚未*明了。但16S的rRNA3’端有一段核苷酸序列与mRNA的前
导序列是互补的,这可能有助于mRNA与核糖体的结合。调控功能
的非编码RNA,其大小长约20~25个核苷酸。成熟的miRNAs是由较
长的初级转录物经过一系列核酸酶的剪切加工而产生的,随后组
装进RNA诱导的沉默复合体,通过碱基互补配对的方式识别靶mRNA
,并根据互补程度的不同指导沉默复合体降解靶mRNA或者阻遏靶
mRNA。
There are 20 kinds of amino acids that make up the protein, and at least 31 species are required according to the codon swing theory
tRNA, but there are only 22 tRNAs in vertebrates. Since 1969, studied
Since a variety of different organisms: such as yeast, E. coli, wheat, mice and a dozen tRNA
Structure, to prove that their base sequence can be folded into clover-shaped secondary structure (Figure 3-
23), and all have the following common features: ① 5 'end with G (most) or C.
② 3 'end of the order to end in CCA. ③ have a guanine ring. ④ there
An anticodon loop, with three exposed bases at the top of the loop, called backflush
The anticodon can pair with complementary codons on the mRNA strand
. ⑤ There is a thymine ring. Also known as ribosomal RNA,
rRNA is the main component of ribosomes. Ribosomes are factories that synthesize proteins. in
In E. coli, rRNA accounts for 75% -85% of total cellular RNA, while tRNA accounts for 15%
mRNA only accounts for 3-5%. RRNAs are typically associated with ribosomal proteins to form ribose
The ribosome. The 30S subunit of E. coli ribosomes has a sedimentation coefficient of 1 molecule
16S rRNA and 21 ribosomal proteins. The 50S subunit consists of two rRNAs (23S
+ 5S) and 34 ribosomal proteins. Eukaryotic ribosomes are more complicated by
More than one rRNA molecule and more proteins. If rRNA from the ribosome
Removed, the ribosome structure collapse occurs. S is the sedimentation coefficient
Sedimentationcoefficient, when one particle is measured by ultracentrifugation
Of the precipitation speed, the speed and the size of the particle diameter proportional to. 5S contains 120 nuclei
The nucleotide, 16S contains 1540 nucleotides, whereas 23S contains 2900 nucleotides. And eukaryotic
There are 4 rRNA organisms, their molecular size is 5S, 5.8S, 18S and 28S, respectively
Do not have about 120, 160, 1900 and 4700 nucleotides. rRNA is single-stranded, it's package
There are unequal amounts of A and U, G and C, but a wide range of double-stranded regions. In the double-stranded region, alkali
Gene hydrogen bonds, the performance of hairpin spiral. The function of rRNA in protein synthesis
Not yet fully understood. However, the 16S rRNA3 'end has a nucleotide sequence that precedes the mRNA
The leader sequence is complementary, which may contribute to the binding of mRNA to ribosomes. Regulatory functions
Of non-coding RNA, the size of about 20 to 25 nucleotides in length. Mature miRNAs are compared
Long primary transcripts are produced by a series of nuclease cleavage processes, followed by the grouping
Into the RNA-induced silencing complex, the target mRNA is recognized by base-pairing
, And depending on the degree of complementarity, silence the silencing complex to direct the target mRNA or repress the target
Translation of mRNA.