鼠抗人CD74单克隆抗体

广州健仑生物科技有限公司

CD74表达于所有的B细胞和某些T细胞亚类（MHC II 阳性），主要是生发中心的淋巴细胞，具有几种异构体（33-41KDa），是HLA-DR的恒定链。此抗体主要用于研究B细胞及其来源的肿瘤，T细胞淋巴瘤很少有表达，染色模式为细胞膜，但可见核旁颗粒状染色，有助于淋巴瘤和白血病的研究。此外，可用于研究非典型纤维黄色肉瘤和恶性纤维组织细胞瘤。

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【产品介绍】

细胞定位：细胞浆/细胞膜

克隆号：LN2

同型：IgG1

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

Percll是一种无毒，惰性，且与生物膜不发生粘附的物质。用它制成的梯度可在室温下放置数星期而梯度的形状不发生丝毫变化。Percoll密度梯度离心已被许多学者用来分离各种动物的抗原抗体，而用于分离早期生精细胞的报道不多。Bucci等［8］用组合酶消化，Percoll等密度梯度离心分离了大鼠的A型精原细胞，所获细胞纯度达到51%。Hofmann等［5］用Percoll不连续密度梯度离心，从10d小鼠睾丸分离生殖细胞，结果精原细胞主要分布于密度为1.030的Pecoll梯度中。但文中没有报告所获细胞的纯度。
本实验结果表明，11%～19%Percoll梯度之间主要为死细胞及细胞碎片19%～27%及35%～43%Percoll梯度之间主要是大量支持细胞和少量管周肌样细胞。精原细胞主要分布于27%～35%Percoll梯度间。电镜观察表明，该带中大部分细胞的形态结构特征与相同日龄的小鼠睾丸切片上精原细胞的形态结构*。该带细胞接种后，根据精原细胞与睾丸体细胞贴壁速度快慢的差异，利用选择性贴壁法，待睾丸体细胞开始贴壁极化而精原细胞仍然悬浮时(体外培养约3～4h)，将其进一步纯化后另行培养；待其贴壁后，依据其形态学特征进行计数，结果精原细胞的纯度平均达到68.76%，高于Bucci等的分离效果。本实验中，支持细胞在3个梯度中都有大量分布，可能与幼年鼠支持细胞非常丰富且其大小很不*有关。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】     欧

【】 
【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

Percll is a non-toxic, inert substance that does not adhere to biofilms. Gradients made with it can be left at room temperature for several weeks without a gradual change in the shape of the gradient. Percoll density gradient centrifugation has been used by many researchers to isolate antigen-antibody of various animals, and few reports have been made on the isolation of early spermatogenic cells. Bucci et al [8] digested by combinatorial enzymes, Percoll density gradient centrifugation of rat spermatogonia type A, the purity of cells obtained 51%. Hofmann et al [5] used Percoll discontinuous density gradient centrifugation to separate germ cells from the 10-day mouse testis. As a result, spermatogonia were mainly distributed in a Pecoli gradient with a density of 1.030. However, the purity of the cells obtained is not reported in the text.
The results of this experiment show that between 11% and 19% of the Percoll gradients are predominantly a large number of supporting cells and few peritubular myoidoid cells between 19% to 27% of dead cells and cell debris and 35% to 43% of the Percoll gradient. Spermatogonia mainly distributed in 27% ~ 35% Percoll gradient. Electron microscopy showed that the morphology of most cells in the band was consistent with that of spermatogonia on the testicular sections of mice of the same age. After the cells are inoculated, the spermatogonia begin to attach to the parietal polarization and the spermatogonium still remain in suspension according to the difference of the adherent rate between spermatogonia and testicular somatic cells (in vitro culture for about 3 ~ 4h). After further purification, the cells were cultured separay. After being adhered, the purity of spermatogonia was 68.76% on average, which was higher than that of Bucci. In this experiment, the supporting cells in a large number of three gradient distribution, may be very rich in juvenile mouse supporting cells and its size is inconsistent.
First, the principle
Living cells contain LDH in their cytoplasm. Under normal circumstances, LDH can not penetrate the cell membrane, when the cells are NK cell-killing, LDH release to the extracellular. LDH dehydrogenates lithium lactate, which in turn reduces NAD to NADH, which in turn reduces Iodonitetrazolyltetrazolium (INT) via the dehydro-phenazine dimethyl ester sulfate (PMS), which undergoes H + reduction Magenta A month like compounds. 490nm colorimetric assay on a microplate reader.
Second, equipment and materials
A microplate reader, a microplate reader, a microplate reader, a YP-1 cell, a Hank's solution (pH 7.2 to 7.4), a complete RPMI1640 medium, lithium lactate or sodium lactate, nitrotetrazolium chloride (INT) (PMS), NAD, 0.2 mol / L Tris-HCl buffer (pH 8.2), 1% NP40 or 2.5% Triton