鼠抗人CD71单克隆抗体

广州健仑生物科技有限公司

转铁蛋白受体CD71在胎盘合体滋养细胞、肌细胞、肝细胞、精母细胞、幼稚红细胞等有高水平表达，其中在幼稚红细胞早期至正常红细胞的过渡期表达zui高，随着红细胞的成熟，转铁蛋白受体CD71表达量随之下降。由于幼稚红细胞中CD71的特性表达使CD71抗体成为一个有价值的作为骨髓中幼稚红细胞成分的参考依据。CD71抗体在骨髓组织中特异性地标记幼稚红细胞，而成熟红细胞，和其它造血细胞几乎不表达CD71，所以，免疫组织化学检测CD71抗体是红血球性白血病、良性红细胞增生性紊乱和骨髓发育不良症的重要参考依据。

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【产品介绍】

细胞定位：细胞浆/细胞膜

克隆号：MRQ-48

同型：IgG1

适用组织：石蜡/冰冻

阳性对照：骨髓

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

讨论
1.小鼠生殖细胞单细胞悬液制备的影响因素
良好单细胞悬液的制备必须首先考虑所用小鼠的日龄。因为随动物日龄的增加，精原细胞开始分化形成各级生殖细胞，其数目及所占生殖细胞的比例都逐渐下降，会给分离纯化增加困难。在成年小鼠的睾丸中，A型精原细胞只占所有精原细胞的1.25%，占未分化精原细胞的10.6%，仅占所有生殖细胞的0.03%［5］。因而用来分离精原细胞的动物的日龄应较小为好。一般小鼠选用生后8d的［2］，但也有人选用生后10d的；大鼠则选用生后9d的［6］。根据我们的前期工作并参考有关报道［2，3］，7～8d小鼠的生精上皮内基本只有A型精原细胞和支持细胞，其他各级生精细胞尚未发育形成，理论上可获得zui大量的精原细胞。
其次，采用适当的程序及消化方法也是制备良好单细胞悬液的关键环节之一。从小鼠体内取出睾丸之后，我们先用尖镊仔细去除脂肪垫及睾丸白膜，将曲细精管在PBS中吹散，尽量将间质吹打成单细胞及组织碎片。通过静置或低速离心，将其去除，获得较为纯粹的曲细精管段。然后采用两次较短时间的胶原酶消化，将没有消散的睾丸间质消散并释放大部分管周肌样细胞，zui大程度地减少了间质组织及管周肌样细胞的污染。zui后采用胰蛋白酶、EDTA复合消化液消化，释放支持细胞和精原细胞。实验结果表明，这种程序性的消化过程能有效地分离到较为纯粹的曲细精管段并制备出较高产量和活率的单细胞悬液，同时确保绝大部分间质成分，管周肌样细胞及睾丸外细胞的去除。
2.Percoll不连续密度梯度的分离效果

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】     欧

【】 
【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

discuss
1 mouse germ cell single cell suspension preparation of the influencing factors
The preparation of a good single cell suspension must first consider the age of the mice used. Because with the increase of animal age, spermatogonial cells begin to differentiate to form germ cells at all levels, the absolute number and proportion of germ cells are gradually decreased, will increase the difficulty of separation and purification. In testis of adult mice, type A spermatogonia account for only 1.25% of all spermatogonia, accounting for 10.6% of undifferentiated spermatogonia, accounting for only 0.03% of all germ cells [5]. Therefore, the animal used to isolate spermatogonia should be as small as possible. General mice selected 8d after birth [2], but also some people choose after birth 10d; rats were selected 9d after birth [6]. According to our previous work and with reference to relevant reports [2, 3], there are only type A spermatogonia and supporting cells in the seminiferous epithelium of 7 ~ 8 days mice, and other spermatogenic cells at all levels have not yet developed and are theoretically available The largest amount of spermatogonia.
Second, using proper procedures and digestion methods is also one of the key steps in preparing a good single cell suspension. After removing the testis from the body of the mouse, we carefully remove the fat pad and the testicular white membrane with sharp tweezers, blow the seminiferous tubules in PBS, and blow the interstitial into single cells and tissue fragments as much as possible. By standing or low-speed centrifugation, it is removed to obtain a more pure seminiferous tubule. Then using two shorter collagenase digestion, dissipate the testicular interstitium and release most of the peritubular myeloid cells, to minimize the interstitial and peritubular myoid-like cell contamination. Finally, trypsin, EDTA digestion of digestion, the release of supporting cells and spermatogonia. The experimental results show that this process of digestion can effectively separate the more pure seminiferous tubules and produce a single cell suspension with higher yield and viability while ensuring that most interstitial components, Like cells and testicular cells removed.
2.Percoll discrete density gradient separation effect