CD68巨噬细胞（鼠单克隆抗体）

广州健仑生物科技有限公司

CD68 是一种分子量为110KDa 的糖蛋白，与溶酶体颗粒有关。此抗体可研究人体许多组织中的巨噬细胞，包括脾脏、肠固有层、肺泡和骨髓中的枯否细胞和巨噬细胞，外周血的单核细胞也呈阳性反应，同时也可以研究骨髓前体细胞和外周血粒细胞。近年来有文献报道，CD68也可以作为组织细胞的标志物。主要用于巨噬细胞方面的研究以及急慢性髓样白血病和组织细胞来源的肿瘤如恶性纤维组织细胞瘤的研究。

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【产品介绍】

细胞定位：细胞浆/细胞膜

克隆号：KP-1

同型：IgG1/K

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（柠檬酸）

抗体孵育时间：30-60min

CD68巨噬细胞（鼠单克隆抗体）

经Percoll密度梯度离心，细胞主要在相邻梯度的界面处形成细胞带。如表2所示，分布在11%～19%Percoll梯度间的主要是细胞间质、细胞碎片及少量死细胞，记为1带；分布在19%～27%percoll梯度间的细胞较少，平均密度为2.35×105个／ml，记为2带；分布在27%～35% Percoll梯度间的细胞zui多，平均密度达1.58×106，记为3带；分布在35%～43% Percoll梯度间的细胞也较少，平均密度为2.68×105，记为4带。统计学分析显示，2、3、4带中细胞分布差异极显著(P<0.01)，其中3带与2、4带之间相比差异极为显著(P<0.01)，2、4带之间相比差异不显著(P>0.05)。
3.分离细胞的形态学鉴定
电镜观察显示，分离细胞圆形或卵圆形，胞质中除可见有少量具有板层状嵴的线粒体外其他细胞器不发达；核质比较大，核大呈圆形，常染色质细密均匀，异染色质少，呈块状附着于核膜内面或散在于核内。其形态结构特征与7～8d小鼠睾丸切片上的精原细胞**(图1)。
4.精原细胞的分离纯度
1带中主要为死细胞及细胞碎片；2、4带中细胞接种后，大部分细胞延展后伸出突起，从形态学判定，主要为支持细胞及少量管周肌样细胞。主要收集3带中细胞，接种培养约3～4h，其中的支持细胞开始贴壁而精原细胞尚未贴壁，此时进行精原细胞的纯化；纯化细胞体外培养约7～8h后，绝大部分支持细胞已贴壁，大部分精原细胞开始贴壁，此时进行细胞计数。结果如表4所示，3带中精原细胞的纯度平均达到68.76%。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】     欧

【】 
【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

After Percoll density gradient centrifugation, the cells formed cell strands predominantly at adjacent gradient interfaces. As shown in Table 2, interstitial cells, cell debris and a small number of dead cells distributed between 11% and 19% of Percoll gradient were recorded as a band. Fewer cells distributed between 19% and 27% percoll gradients, The average density was 2.35 × 105 cells / ml, which was recorded as 2 bands. The cells distributed in 27% -35% Percoll gradient had the highest average density of 1.58 × 106, which was recorded as 3 bands. The distribution was between 35% and 43% of Percoll gradient Between the cells less, the average density of 2.68 × 105, recorded as 4 band. Statistical analysis showed that there was significant difference in the distribution of cells in 2,3,4 band (P <0.01), among which the difference between 3 band and 2,4 band was extremely significant (P <0.01) Compared with the difference was not significant (P> 0.05).
3. Isolation of cells morphological identification
Electron microscopy showed that the cells isolated round or oval, in addition to the cytoplasm can be seen in a small amount of mitochondria with lamellar ridges other underdeveloped organelles; relatively large nuclear, nuclear large circular, dense chromatin, Heterochromatin less, was attached to the nucleus inside the patch or scattered in the nucleus. The morphological features of the spermatogonia were consistent with the spermatogonia in 7-8 day mouse testis sections (Figure 1).
Separation of spermatogonia purity
1 with dead cells and cell debris in the band; 2,4 cells with inoculation, the majority of cells after the extension of the protruding process, from the morphological determination, mainly for supporting cells and a small amount of tubular muscle-like cells. Mainly collected cells with 3, inoculation culture of about 3 ~ 4h, in which the supporting cells began to adhere to the spermatogonia have not yet attached (Figure 2), then spermatogonia purified; purified cells cultured in vitro about 7 ~ 8h After most of the supporting cells have been adherent, most of the spermatogonia began to attach (Figure 3), at this time for cell counting. As shown in Table 4, the purity of the spermatogonia in the third band reached an average of 68.76%.