CD45(LCA)白细胞共同抗原

广州健仑生物科技有限公司

白细胞共同抗原主要位于白细胞表面，包括 T、B 淋巴细胞、多形核白细胞、单核细胞等，因此是区别淋巴瘤/白血病和非造血组织肿瘤如未分化小细胞癌、小圆细胞肉瘤的特异性参考依据。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

欢迎咨询

欢迎咨询

【产品介绍】

细胞定位：细胞膜

克隆号：2B11&PD7/26

同型：IgG1/K

适用组织：石蜡/冰冻

阳性对照：扁桃体/小细胞淋巴瘤

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

CD45(LCA)白细胞共同抗原

理想细胞转染方法，应该具有转染效率高、细胞毒性小等优点。病毒介导的转染技术，是目前转染效率zui高的方法，同时具有细胞毒性很低的优势。但是，病毒转染方法的准备程序复杂，常常对细胞类型有很强的选择性，在一般实验室中很难普及。其它物理和化学介导的转染方法，则各有其特点。
需要指出的一点，无论采用哪种转染技术，要获得*的转染结果，可能都需要对转染条件进行优化。影响转染效率的因素很多，从细胞类型、细胞培养条件和细胞生长状态，到转染方法的操作细节，都需要考虑。
一、细胞传代
1. 试验准备：200ul/1mlTip头各一盒(以上物品均需高压灭菌)，酒精棉球，废液缸，试管架，微量移液器，记号笔，培养皿，离心管。
2. 弃掉培养皿中的培养基，用1ml的PBS溶液洗涤两次。
3. 用Tip头加入1ml Trypsin液，消化1分钟(37℃，5%CO2 )。用手轻拍培养瓶壁，观察到细胞*从壁上脱落下来为止。
4. 加入1ml的含血清培养基终止反应。
5. 用Tip头多次吹吸，使细胞*分散开。
6. 将培养液装入离心管中，1000rpm离心5min。
7. 用培养液重悬细胞，细胞计数后选择0.8X106个细胞加入一个35mm培养皿。
8. 将合适体积*培养液加入离心管中，混匀细胞后轻轻加入培养皿中，使其均匀分布。
9. 将培养皿转入CO2培养箱中培养，第二天转染。

CD45(LCA)白细胞共同抗原

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】     欧

【】 
【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

The ideal cell transfection method should have the advantages of high transfection efficiency and low cytotoxicity. Virus-mediated transfection is currently the most efficient transfection method, with low cytotoxicity advantages. However, the procedure for preparation of virus-based transfection is complex and is often highly selective for cell types and is not readily available in the general laboratory. Other physical and chemical-mediated transfection methods have their own characteristics.
It should be pointed out that no matter what kind of transfection technique is used, the optimal transfection results may need to be optimized for transfection conditions. Factors that affect transfection efficiency are numerous and need to be taken into account in terms of cell type, cell culture conditions, and cell growth status, as well as the operational details of the transfection method.
First, the cell passage
1. Test Preparation: 200ul / 1mlTip each one box (above items are autoclaved), alcohol cotton balls, waste tanks, test tube rack, micropipette, marker, petri dishes, centrifuge tubes.
2. Discard the culture medium in the Petri dish and wash twice with 1 ml of PBS solution.
3. Add 1 ml of Trypsin solution to Tip for 1 minute (37 ° C, 5% CO2). Raise the bottle wall with a hand and observe that the cell has compley come off the wall.
4. Add 1ml of serum-containing medium to stop the reaction.
Tip Tip repeatedly suction, the cells compley dispersed.
6. The culture medium into the centrifuge tube, 1000rpm centrifuge 5min.
7. Resuspend the cells in culture and select 0.8 × 10 6 cells to add to a 35 mm Petri dish.
8. The appropriate volume of complete medium was added to the centrifuge tube, mix the cells gently added to the Petri dish, to its uniform distribution.
9. Petri dishes into CO2 incubator culture, the next day transfected.