CD35滤泡树突状细胞（鼠单克隆抗体）

广州健仑生物科技有限公司

CD35是一种分子量为160-250kDa的跨膜蛋白，可与补体C3b和C4b结合，故又称补体受体1（CR1）。CD35可作为成熟B细胞的标记物，在红细胞、B细胞、T细胞亚群、单核细胞、嗜酸粒细胞和中性粒细胞中表达。主要用于研究滤泡树突状细胞及其来源的肿瘤。

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【产品介绍】

细胞定位：细胞膜

克隆号：RLB25

同型：IgG2b

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

CD35滤泡树突状细胞（鼠单克隆抗体）

Folin—酚试剂法zui早由Lowry确定了蛋白质浓度测定的基本步骤。以后在生物化学领域得到广泛的应用。这个测定法的优点是灵敏度高，比双缩脲法灵敏得多，缺点是费时间较长，要精确控制操作时间，标准曲线也不是严格的直线形式，且专一性较差，干扰物质较多。对双缩脲反应发生干扰的离子，同样容易干扰Lowry反应。而且对后者的影响还要大得多。酚类、柠檬酸、硫酸铵、Tris缓冲液、甘氨酸、糖类、甘油等均有干扰作用。浓度较低的尿素（0.5%），硫酸纳（1%），硝酸纳（1%），三氯乙酸（0.5%），乙醇（5%），抗原抗体（5%），丙酮（0.5%）等溶液对显色无影响，但这些物质浓度高时，必须作校正曲线。含硫酸铵的溶液，只须加浓碳酸钠—氢氧化钠溶液，即可显色测定。若样品酸度较高，显色后会色浅，则必须提高碳酸钠—氢氧化钠溶液的浓度1~2倍。
进行测定时，加F olin—酚试剂时要特别小心，因为该试剂仅在酸性pH条件下稳定，但上述还原反应只在pH=10的情况下发生，故当Folin一酚试剂加到碱性的铜—蛋白质溶液中时，必须立即混匀，以便在磷钼酸—磷钨酸试剂被破坏之前，还原反应即能发生。
此法也适用于酪氨酸和色氨酸的定量测定。
此法可检测的zui低蛋白质量达5mg。通常测定范围是20~250mg。
（二）试剂与器材
1.试剂
（1）试剂甲：
（A）10克Na2CO3，2克NaOH和0.25克酒石酸钾钠（KNaC4H4O6·4H2O）。溶解于500毫升蒸馏水中。
（B）0.5克硫酸铜（CuSO4·5H2O）溶解于100毫升蒸馏水中，每次使用前，将50份（A）与1份（B）混合，即为试剂甲。
（2）试剂乙：

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

Folin-phenol reagent method was first determined by Lowry the basic steps of protein concentration determination. Later in the field of biochemistry has been widely used. This assay has the advantages of high sensitivity, much more sensitive than the biuret method, the disadvantage is that time is longer, to accuray control the operating time, the standard curve is not strictly a straight line, and less specific, interfering substances than many. Interferences on the biuret reaction interfere with the Lowry reaction. And the impact on the latter is even greater. Phenols, citric acid, ammonium sulfate, Tris buffer, glycine, carbohydrates, etc. have interference. (0.5%), sodium sulphate (1%), sodium nitrate (1%), trichloroacetic acid (0.5%), ethanol (5%), antigen antibody (5%), acetone Such solution has no effect on the color, but the concentration of these substances is high, you must make a calibration curve. Ammonium sulfate-containing solution, just add concentrated sodium carbonate - sodium hydroxide solution, you can color determination. If the sample has a high acidity and a light color after color development, the concentration of sodium carbonate-sodium hydroxide solution must be increased by 1 to 2 times.
Be careful when adding F olin-phenol reagent because the reagent is stable only at acidic pH but the above reduction reaction only occurs at pH = 10. Therefore, when Folin phenol reagent is added to basic Of the copper-protein solution, it must be mixed immediay so that the reduction reaction can take place before the phosphomolybdic acid-phosphotungstic acid reagent is destroyed.
This method is also suitable for the quantitative determination of tyrosine and tryptophan.
This method can detect the minimum amount of protein up to 5mg. The usual measurement range is 20 ~ 250mg.
(B) Reagents and equipment
Reagents
(1) Reagent A:
(A) 10 grams of Na2CO3, 2 grams of NaOH and 0.25 grams of potassium sodium tartrate (KNaC4H4O6.4H2O). Dissolve in 500 ml of distilled water.
(B) 0.5 g of copper sulfate (CuSO4 · 5H2O) was dissolved in 100 ml of distilled water, and 50 parts of (A) and 1 part of (B) were mixed before each use to give reagent A.
(2) Reagent B: