CD23(B细胞)鼠单克隆抗体

广州健仑生物科技有限公司

CD23 是一种分子量为 45KDa的细胞膜表面糖蛋白，是存在于 B细胞表面的低亲和性 IgE 受体。表达于外周血细胞的某些亚群、 B淋巴细胞、淋巴滤泡树突细胞(FDC)、初始淋巴细胞和 EB 病毒转染的 B 淋巴瘤细胞系。慢性淋巴细胞白血病和小淋巴细胞性淋巴瘤瘤细胞也表达CD23，但套细胞淋巴瘤不表达 CD23。

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【产品介绍】

细胞定位：细胞膜

克隆号：MRQ-57

同型：IgG1/K

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

CD23(B细胞)鼠单克隆抗体

测定微生物细胞数量的方法很多，通常采用的有显微镜直接计数法和稀释平板计数法。
直接计数法适用于各种单细胞菌体的纯培养悬浮液，如有杂菌或杂质，则难于直接测定。菌体较大的酵母菌或霉菌孢子可采用血球计数板，一般细菌则采用彼德罗夫·霍泽（Petrof Hausser）细菌计数板。两种计数板的原理和部件相同，只是细菌计数板较薄，可以使用油镜观察。而血球计数板较厚，不能使用油镜，计数板下部的细菌难于区分。
血球计数板是一块特制的厚型载玻片，载坡片上有4条槽所构成的3个平台。中间的平台较宽，其中间又被一短横槽分隔成两半，每个半边上面各有一个计数区（图示），计数区的刻度有两种：一种是计数区分为16个大方格，大方格用三线隔开，而每个大方格又分成25个小方格；另一种是一个计数区分成25个大方格，大方格之间用双线分开，而每个大方格又分成16个小方格。但是不管计数区是哪一种构造，它们都有一个共同特点，即计数区都由400个小方格组成（图示）。
计数区边长为1mm，则计数区的面积为1mm2，每个小方格的面积为1/400 mm2。盖上盖玻片后，         
计数区的高度为0.1mm，所以计数区的体积为0.1mm3，每个小方格的体积为1/4000 mm3。
使用血球计数板计数时，先要测定每个小方格中微生物的数量，再换算成每mL菌液（或每g样品）中微生物细胞的数量。
已知：1mL体积＝10 mm×10 mm×10 mm＝1000mm3       
所以：1mL体职应含有小方格数为1000mm3/（1/4000mm3）＝4×106个小方格，即系数K＝4×106。
因此：每mL菌悬液中含有细胞数＝每个小格中细胞平均数（N）×系数（K）×菌液稀释倍数（d）。
六、实验材料
活材料：酿酒酵母（Saccharomyces cerevisiae）斜面菌种或培养液、枯草杆菌（Bacillus subtilis）染色标本片。

CD23(B细胞)鼠单克隆抗体

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

There are many ways to determine the number of microbial cells, usually the direct counting microscope and the dilution plate counting method.
Direct counting method for a variety of single cell suspension of pure culture, such as bacteria or impurities, it is difficult to direct determination. Larger cell strains of yeast or mold spores can be used blood count plate, the general bacteria are used Petrof Hausser bacteria count plate. The principle and components of the two counting plate the same, but the bacterial count plate is thin, you can use oil mirror observation. The blood count plate thicker, can not use the oil mirror, the lower part of the counting plate difficult to distinguish bacteria.
The blood cell counting plate is a special thick glass slide, which contains 3 platforms formed by 4 slots. The middle of the platform wider, the middle was a short groove separated by two halves, each half of each have a counting zone (Figure), there are two counting zone scale: a count is divided into 16 large Checkered, large squares separated by three lines, and each large square is divided into 25 small squares; the other is a count is divided into 25 large square, large squares separated by two lines, and Each big square is divided into 16 small squares. But regardless of the construction of the counting zone, they all have one thing in common: the counting zone consists of 400 small squares (pictured).
Counting area edge length is 1mm, then counting area area of ??1mm2, each small square area of ??1 / 400mm2. After covering the cover glass,
The height of the counting zone is 0.1 mm, so the volume of the counting zone is 0.1 mm3 and the volume of each small square is 1/4000 mm3.
When counting with a hemocytometer, the number of microbes in each small square is determined and then converted into the number of microbial cells per mL of broth (or per gram of sample).
It is known that 1 mL volume = 10 mm × 10 mm × 10 mm = 1000 mm 3
So: 1mL bodywork should contain a small number of squares 1000mm3 / (1 / 4000mm3) = 4 × 106 small squares, the coefficient K = 4 × 106.
Therefore: Number of cells contained in each mL of bacterial suspension = average number of cells in each cell (N) x coefficient (K) x bacterial dilution (d).
Six, experimental materials
Live material: Saccharomyces cerevisiae benthic strain or culture, Bacillus subtilis stained specimen.