CD21(B细胞)兔单克隆抗体

广州健仑生物科技有限公司

CD21 又称 2 型补体受体（complement receptor type 2，CR2）和 EB 病毒受体，是补体激活调节剂家族的一员。CD21主要分布在成熟的 B细胞、淋巴滤泡内的树突细胞、部分 T 细胞。CD21 的功能有促进 细胞增殖，介导 EBV 转化 B 细胞，参与免疫记忆。主要用于滤泡状细胞瘤和免疫补体激活调节的研究。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【产品介绍】

细胞定位：细胞膜/细胞浆

克隆号：EP3093

同型：IgG

适用组织：石蜡/冰冻

阳性对照：扁桃体

抗原修复：热修复（EDTA）

抗体孵育时间：30-60min

CD21(B细胞)兔单克隆抗体

6、化学药剂对微生物的作用
⑴制平板：取无菌平皿3套，将已熔化并冷却至50℃左右的抗原抗体蛋白胨琼脂培养基按无菌操作法倒入平皿中，使冷凝成平板。
⑵制备菌悬液：取无菌水3支，用接种环分别取大肠杆菌、金黄色葡萄球菌和枯草杆菌各1～2环接入无菌水中，充分混匀，制成菌悬液。
⑶接种：用无菌吸管分别吸取已制好的菌悬液0.1mL接种于平板上，用无菌玻璃刮铲涂匀。注意做好标记。
⑷浸药：将灭菌滤纸片浸入供试药剂中。
⑸加药剂：用无菌镊子夹取浸药滤纸片，注意把药液沥干，分别平铺于同一含菌平
板上，注意药剂之间勿互相沾染并在平皿背面做好标记。
⑹培养：将平皿置于28℃下培养48～72h后观察结果。
实验原理
微生物细胞的大小是微生物重要的形态特征之一，由于菌体微小，只能在显微镜下测量。用于测量微生物细胞大小的工具有目镜测微尺和镜台测微尺。
目镜测微尺（图示）是一块圆形玻片，在玻片中央把5mm长度刻成50等分，或把10mm长度刻成100等分。测量时，将其放在接目镜中的隔板上，此处正好与物镜放大的中间物像重迭，用于测量经显微镜放大后的细胞物象。由于不同目镜、物镜组合的放大倍数不相同，目镜测微尺每格实际表示的长度也不一样，因此目镜测微尺测量微生物大小时须先用置于镜台上的镜台测微尺校正，以求出在一定放大倍数下，目镜测微尺每小格所代表的相对长度。镜台测微尺（图示）是中央部分刻有精确等分线的载玻片，一般将1mm等分为100格，每格长10μm即0.01mm，是专门用来校正目镜测微尺的。校正时，将镜台测微尺放在载物台上，由于镜台测微尺与细胞标本是处于同一位置，都要经过物镜和目镜的两次放大成像进入视野，即镜台测微尺随着显微镜总放大倍数的放大而放大，因此从镜台测微尺上得到的读数就是细胞的真实大小，所以用镜台测微尺的已知长度在一定放大倍数下校正目镜测微尺，即可求出目镜测微尺每格所代表的实际长度，然后移去镜台测微尺，换上待测标本片，用校正好的目镜测微尺在同样放大倍数下测量微生物细胞大小。

CD21(B细胞)兔单克隆抗体

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

6, the role of chemical agents on microorganisms
⑴ system plate: Take three sets of sterile dishes, will have been melted and cooled to about 50 ℃ antigen antibody peptone agar medium according to aseptic method into the plate, so that the condensation into the plate.
⑵ Preparation of bacterial suspension: take sterile water 3, with the inoculation loop were taken Escherichia coli, Staphylococcus aureus and Bacillus subtilis 1 ~ 2 loop access to sterile water, mix well to make bacterial suspension.
(3) Inoculation: Use sterile pipette respectively to absorb 0.1mL of prepared bacterial suspension and inoculate them on the plate, and spread them evenly with a sterile glass spatula. Take care to mark it.
⑷ dip medicine: sterile filter paper immersed in the test agent.
⑸ add medicine: with sterile tweezers grip leaching filter paper, pay attention to drain the liquid, respectively, tiled in the same bacteria level
On the board, pay attention to the drug do not contaminate each other and mark the back of the plate.
⑹ culture: the plate was placed at 28 ℃ for 48 ~ 72h after the observation.
Experimental principle
Microbial cell size is one of the important morphological characteristics of microorganisms, due to the tiny cells can only be measured under a microscope. Tools for measuring microbial cell size include eyepiece micrometer and stage micrometer.
The eyepiece micrometer (pictured) is a round slide with a length of 5 mm engraved into the center of the slide, or a 10 mm length cut into 100 equal parts. When measured, it is placed on the partition in the eyepiece, where it overlaps with the magnified intermediate image of the objective to measure the magnified cellular image. Because different eyepiece, objective combination of magnification is not the same, eyepiece micrometer ruler each grid actually expressed length is not the same, so the eyepiece micrometer ruler to measure the size of the microorganism must be placed on the stage with a microscope micrometer ruler to Obtained at a certain magnification, eyepiece micrometer scale each cell represents the relative length. The microscope micrometer scale (pictorial) is a special glass slide engraved with precise bisector in the central part. It usually divides 1mm equally into 100 cells, each 10μm long or 0.01mm, which is designed to correct the eyepiece micrometer . Calibration, the microscope micrometer ruler placed on the stage, as the stage micrometer ruler and cell specimens are in the same position, both through the objective lens and eyepiece magnification into the field of vision two times, that mirror stage micrometer with the microscope The total magnification of amplification and amplification, so the micrometer from the stage to get the reading is the true size of the cell, so the known length of the mirror with a micrometer scale known magnification correction eyepiece micrometer, you can find the eyepiece Measure the actual length of each cell represented by the micrometer and then remove the micrometer from the stage and place the specimen under test with a calibrated eyepiece micrometer to measure microbial cell size at the same magnification.