Recombinant Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)重组蛋白

[ PROPERTIES ]

Residues:Thr701~Pro898,with two N-terminal Tags, His-tag and T7-tag.

Host:E. coli

Subcellular Location: Cell membrane;Single-pass type I membrane protein.

Purity:>95%

Endotoxin Level:<1.0EU per 1μg(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point:6.6

Predicted Molecular Mass:25.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4..

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQM GRGSEF- TSTIIRGLNA STRYLFRMRASIQGLGDWSN TVEESTLGNG LQAEGPVQES RAAEEGLDQ LILAVVGSVS ATCLTILAALLTLVCIRRSC LHRRRTFTYQ SGSGEETILQ FSSGTLTLTR RPKLQPEPLS YPVLEWEDITFEDLIGEGNF GQVIRAMIKK DGLKMNAAIK MLKEYASEND

[  IDENTIFICATION  ]

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