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Use RAPID'E.coli O157:H7 Agar for the detection, isolation, and presumptive identification of E. coli O157:H7 in food products. Results should be confirmed by standard reference methods appropriate for the food type being tested. Confirmation using latex is also validated for colonies isolated from RAPID'E.coli O157:H7 agar. This product includes 100 g of dehydrated medium. Supplements of novobiocin (10 mg/L) and potassium tellurite (0.8 mg/L) must be added to this medium. Features and Benefits Detection on a single plate Typical (sorbitol-/β-glucuronidase-) and atypical (β-glucuronidase+) E. coli O157:H7 colonies are dark blue or black Other atypical (sorbitol+) E. coli O157:H7 colonies are blue to turquoise blue 99% specificity demonstrated by testing 50 strains of E. coli O157:H7 and 35 other strains Selectivity is increased by the addition of novobiocin and other selective agents such as potassium tellurite Results with RAPID'E.coli O157:H2 Medium in ≤48 hr Validations Certified NF VALIDATION according to the ISO 16140 standard and validated by the AOAC. Related Products STEC Enrichment Broth, dehydrated, 500 g (3564001) and 5 kg (3564002) Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready-to-use (3555426) Modified Tryptic Soy Broth (mTSB) Base, dehydrated (3564426) Novobiocin, powder (3564610) iQ-Check? E. coli O157:H7 Real-Time PCR Kit (3578114) iQ-Check STEC VirX Real-Time PCR Kit (3578139) for detection of stx1-stx2 and eae virulence genes iQ-Check STEC SerO Real-Time PCR Kit (3578140) for detection of six O serotypes (O26, O111, O45, O145, O121, and O103) plus E. coli O157:H7