hCEACAM5/4T1 hCEACAM5/4T1 hCEACAM5/4T1
| I. Background | |||
| CEACAM 家族由膜结合糖蛋白和分泌性糖蛋白组成。膜结合糖蛋白通过糖磷脂酰肌醇 （GPI）或跨膜结构域锚定在细胞表面。具有 GPI 锚定的成员主要包括 CEACAM5、 CEACAM6、CEACAM7 和 CEACAM8。CEACAM 5 广泛表达于内皮细胞、上皮细胞、免 疫细胞等多种细胞类型表面。CEACAM 5 在正常组织内主要作为细胞粘附分子，介导同质 和异质性黏附。CEACAM5 编码的蛋白质可以调节细胞分化、凋亡和细胞极性，从而促进 肿瘤的发展。因此，它也被用作胃肠道癌症的临床生物标志物。在癌细胞中，CEACAM5 主要在上皮肿瘤细胞表面高表达，包括结直肠癌、胃癌、胰腺癌、胆囊癌、肺腺癌、小细 胞肺癌、乳腺癌、膀胱癌、卵巢癌等。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | hCEACAM5 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+2 μg/ml puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | |||
Figure 1. Recombinant hCEACAM5/4T1 stably expressing hCEACAM5. | |||
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