hPTK7/4T1 hPTK7/4T1 hPTK7/4T1
| I. Background | |||
| PTK7 是一种缺乏催化活性的受体蛋白激酶，隶属于蛋白激酶家族，因最早在 结肠癌中被发现，故又被称为 cck-4。作为 Wnt/β-catenin 和 Wnt/PCP 信号通路的常见参与 者，PTK7 对这两种信号通路均有促进或抑制作用。PTK7 能与相应配体结合（即二聚化）、 并自身磷酸化激活下游一系列级联传递并调控细胞增殖、分化及凋亡。 PTK7 基因存在组 织特异性，在多种肿瘤中表达水平升高，其高表达于胃癌、结直肠肿瘤、乳腺癌、卵巢癌 等。 而在子宫颈癌、脂肪肉瘤、血液肿瘤中 ，PTK7 呈现为低表达。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | hPTK7 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+2 μg/ml puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | |||
Figure 1. Recombinant hPTK7/4T1 stably expressing hPTK7. | |||
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