SKBR3-Luc示踪细胞株 CBP30203L | |
| I. Introduction | |
| Host Cell: | SK-BR-3 |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | McCoy's 5a+10%FBS+1ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Mammary gland/breast; derived from metastatic site: pleural effusion |
| Disease: | Adenocarcinoma |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.. The number of Luminescence signal is 511±21(Mean±SD) on SK-BR-3 cells while 1184362±47471(Mean±SD) on SK-BR-3-Luc cells. | |
| IV. Stablity Test | |
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Figure. Stablity test results.SK-BR-3-Luc cells showed at least 32 passage stability with similar luminescence unit. | |
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