| CBP30201L | |
| I. Introduction | |
| Host Cell: | C32 |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+2ug/ml puromycin We strongly suggest to purchase the complete medium from Cobioer. |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | skin |
| Disease: | melanoma, amelanotic |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
| |
Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.. The number of Luminescence signal is 545±3(Mean±SD) on C32 cells while 20130230±1051734(Mean±SD) on C32-Luc cells. | |
| IV. Stablity Test | |
| |
Figure. Stablity test results. C32-Luc cells showed at least 32 passage stability with similar luminescence unit. | |
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