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15021010459

干細(xì)胞無(wú)血清培養(yǎng)基

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[供應(yīng)]TAKARA Cellartis®干細(xì)胞研究產(chǎn)品-小鼠ES細(xì)胞神經(jīng)分化培養(yǎng)基 RHB-A&

貨物所在地:重慶重慶市
更新時(shí)間:2025-05-21 09:37:00
有效期:2025年5月21日 -- 2025年11月21日
已獲點(diǎn)擊:139

產(chǎn)品簡(jiǎn)介
熱烈祝賀華雅生物成為TAKARA Cellartis®干細(xì)胞研究產(chǎn)品的全國(guó)代理商
TAKARA Cellartis®干細(xì)胞研究產(chǎn)品研發(fā)團(tuán)隊(duì)上百人,憑借20多年干細(xì)胞以及相關(guān)培養(yǎng)基研發(fā)經(jīng)驗(yàn),研發(fā)并上市干細(xì)胞研究相關(guān)的多種產(chǎn)品,包括干細(xì)胞培養(yǎng)基,IPS干細(xì)胞系,干細(xì)胞分化細(xì)胞以及培養(yǎng)基,干細(xì)胞檢測(cè)抗體等;產(chǎn)品品種豐富,*,具有配方和*的產(chǎn)品特質(zhì).
詳細(xì)介紹
Neural Stem Cell Culture Media
RHB-A和RHB-Basal是兩款無(wú)血清、成分*確定的培養(yǎng)基,用于人類或小鼠神經(jīng)干細(xì)胞(NS)的定向分化獲取、維持培養(yǎng)、擴(kuò)增、誘導(dǎo)分化成神經(jīng)細(xì)胞等用途。RHB-A可用來(lái)培養(yǎng)擴(kuò)增貼壁的人或小鼠的神經(jīng)干細(xì)胞。在RHB-A中培養(yǎng)的神經(jīng)干細(xì)胞保持著穩(wěn)定的神經(jīng)干細(xì)胞分化發(fā)育成為成熟的神經(jīng)細(xì)胞的能力 (1-3) 。另外,RHB-A還用來(lái)定向分化小鼠胚胎干細(xì)胞(mouse ES)至神經(jīng)前體細(xì)胞 (neural precursors) (4-6)。
RHB‐Basal不包含生長(zhǎng)因子或是神經(jīng)細(xì)胞相關(guān)的添加物。因此,可以RHB‐Basal為基礎(chǔ),通過(guò)添加客戶認(rèn)為需要的添加物來(lái)開(kāi)發(fā)適合客戶的特殊類型細(xì)胞的培養(yǎng)基。
 
■ 產(chǎn)品特點(diǎn)
· RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent 
   human and mouse NS cells
· RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be 
   customized by the addition of supplements 
 
■ 產(chǎn)品應(yīng)用
· Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
· Maintenance and propagation of adherent mouse and human NS cells
· Differentiation of mouse and human NS cells into functional neurons 
· Differentiation of mouse ES cells to neuronal precursors 
· Refer to the Data Sheet for additional examples of use
 

■ 產(chǎn)品詳情

 
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).
 
參考文獻(xiàn):
1. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. 
    Nature Biotechnology 21:183-186.
2. Conti L, et al. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell. 
    PLoS Biology 3(9):e283.
3. Pollard SM, et al. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex 16:112-120.
4. Diogo MM, et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells.
    Biotechnology and Applied Biochemistry 49:105-112.
5. Pollard SM, et al. (2008) Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors 
    and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience 38:393:403.
6. Sun Y, et al. (2008) Long-term tripotent differentiation capacity of human neural stem (NS)cells in adherent culture. 
    Molecular and Cellular Neuroscience 38:245-258.
7. Pollard SM, et al. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are 
    suitable for chemical and genetic screens. Cell Stem Cell 4:568-580.
8. Abranches E, et al. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis 
    in the embryo. PLoS ONE 4(7): e6286.
9. Fernandes et al. (2010) Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells.
    Biotechnology and Bioengineering 106: 260–270.
10. Fernandes, T.G., et al. (2010) Different stages of pluripotency determine distinct patterns of proliferation, metabolism, 

重慶市華雅干細(xì)胞技術(shù)有限公司主營(yíng)產(chǎn)品:Lonza支原體檢測(cè)試劑盒,Stemcell胚胎干細(xì)胞培養(yǎng)基,StemRD生長(zhǎng)分化因子 化工儀器網(wǎng) 設(shè)計(jì)制作,未經(jīng)允許翻錄必究.Copyright(C) http://www.mtfinger.com, All rights reserved.

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