Ni-NTA-Nanogold，镍修饰球形纳米金

Ni-NTA-Nanogold，镍修饰球形纳米金

Ni-NTA-Nanogold，镍修饰纳米金，镍功能化纳米金检测蛋白，Ni-Gold Nanoparticles

西安瑞禧生物科技有限公司可以提供一种Ni-NTA-Nanogold镍修饰纳米金颗粒，该产品可以用于为多聚组氨酸检测或定位（其他）标签的融合蛋白的一款产品。

产品列表：

1.8 nm Ni-NTA-Nanogold

1.8 nm gold particles functionalized with nickel (II) nitraloacetic acid (NTA) chelates. Use for localizing and detecting polyhistidine-tagged targets in protein complexes, cells or tissues. Smaller than antibody probes.

5 nm Ni-NTA-Nanogold

5 nm gold particles functionalized with nickel (II) nitraloacetic acid (NTA) chelates. Use for localizing and detecting polyhistidine-tagged targets in protein complexes, cells or tissues.

10 nm Ni-NTA-Nanogold

10 nm gold particles functionalized with nickel (II) nitraloacetic acid (NTA) chelates. Use for localizing and detecting polyhistidine-tagged targets in protein complexes, cells or tissue

产品信息如下：

PRODUCT INFORMATION

Product Name: 1.8 nm Ni-NTA-Nanogold®

Catalog Number: 2080

Appearance: Brown solution

Size: 3 mL

Storage: Upon receipt store product at 2-8°C. Product is shipped at ambient temperature.

1.8 nm Ni-NTA-Nanogold® is designed for detection or localization of histidine (his) -tagged fusion proteins using electron microscopy, light microscopy, blotting or other detection method. Using 1.8 nm Ni-NTA-Nanogold®, his-tagged fusion proteins originating from any of a variety of expression vectors can be labeled under both non-denaturing and denaturing conditions. The labeled his-tagged fusion proteins can be visualized by microscope or eyes when used with or without gold or silver enhancement reagents, such as GoldEnhance EM (Catalog number 2113), GoldEnhance LM (Catalog number 2112), HQ Silver (Catalog Number 2012), or LI Silver (Catalog number 2013).

INTRODUCTION：

The His-tag, consisting of five to ten consecutive histidine residues, has been used for purification of proteins by immobilized metalion affinity chromatography (IMAC).1-3 The use of a His tag has several advantages. There is minimal addition of extra amino acids to the recombinant proteins. The small histidine tail is poorly immunogenic and usually does not interfere with protein folding. Histagged proteins can have an extremely high affinity for metal ions (Ka=1013 M).1,4-6 This affinity for metal ions allows for the detection of the fusion proteins using Ni-NTA (nickel-nitrilotriacetic acid) Nanogold® (Catalog Number 2080). 1.8 nm Ni-NTA-Nanogold® consists of a 1.8 nm Nanogold particle with multiple nickel-nitrilotriacetic acid functionalities incorporated into the ligands on the surface of gold particles. Each Ni2+ coordinates with one nitrilotriacetic acid and two histidines from the fusion proteins, and forms a stable complex (Figure 1). The his-tagged fusion proteins are therefore labeled, and detected by electron microscopy, light microscopy or blotting, when used with or without gold or silver enhancement reagents.

PRODUCT INFORMATION：

This product is supplied as a brown colored liquid at a concentration of 10 nmol/ml of 1.8 nm Ni-NTA-Nanogold® in 50 mM MOPs, pH 7.9. No additional stabilizer or preservative is included. If a sterile solution is needed, filter the product with a 0.2 um cellulose acetate membrane filter. As supplied, this product is stable at least 1 year when stored at 2-8°C.

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西安瑞禧生物科技有限公司

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