一氧化氮广泛分布于生物体内各组织中，特别是神经组织中。它是一种新型生物信使分子。NO是一种极不稳定的生物自由基，分子小，结构简单，常温下为气体，微溶于水，具有脂溶性，可快速透过生物膜扩散，生物半衰期只有3-5秒，其依赖于一氧化化氮合成酶(nitric oxide synthase , NOS)并在心、脑血管调节、神经、免疫调节等方面有着十分重要的生物学作用。

一氧化氮比色分析试剂盒提供了一个只需要两部，便可准确便捷的检测检测亚硝酸盐离子和硝酸盐离子浓度的方案。*步，硝酸盐离子被硝酸盐还原酶转化成亚硝酸盐离子，然后，使用Griess试剂将亚硝酸盐转化成深紫色的含氮化合物。该含氮化合物内发色团的量反应了检测样本内NO的总量。该试剂盒检测灵敏度为0·1nmole 亚硝酸盐/孔或1μM亚硝酸盐。该一氧化氮荧光分析试剂盒可用于哺乳动物细胞组织裂解液、细胞培养上清、血浆、血清、尿液以及组织匀浆内一氧化氮含量的检测。

如需了解人ELISA试剂盒价格是否有折扣、*或是否有调整,请咨询我司业务人员:

,.

Kit Summary:
• Detection method- Absorbance （540 nm）
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

Features & Benefits:
• Simple two-step procedure; takes only ~1-1.5 hours
• Fast and convenient
• BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite.

Kit components:
• Assay Buffer
• Enzyme cofactor
• Enhancer
• Nitrate Reductase
• Nitrate Standard
• Nitrite Standard
• Griess Reagent R1
• Griess Reagent R2
• Microtiter Plate
• Plate Cover

Description:
Nitric oxide （NO） plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production. BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accuray reflects nitric oxide amount in samples. The detection limit of the assay is approximay 0.1 nmole nitrite/well, or 1 µM.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.