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Applied BioProbes 推出一系列核酸定量检测试剂盒iQuant™ Nucleic Acid Quantitation Kits。这些试剂盒采用Applied BioProbes开发的新型核酸染料与DNA或RNA特异性结合产生荧光，从而实现精确而灵敏的荧光定量。与传统用NanoDrop测吸收的方法相比，iQuant™ Nucleic Acid Quantitation Kits具有灵敏性高、线性范围宽、定量更精确等优点，并且与Life Technologies 的 Qubit® 荧光仪可以*兼容使用。

产品特点

◆灵敏度高：比传统的NanoDrop高100倍的灵敏度；
◆线性范围宽：比传统的NanoDrop拥有更宽的线性区间；
◆特异性好：可分别实现对DNA或RNA定量；
◆操作简单：mix-and-read format;
◆适用性广：可使用荧光酶标仪，也可兼容荧光仪比如Qubit®，Quantus™。?

DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequence, PCR, Cloning, and DNA transfections (Figure 1).

Figure 1. Nucleic acid research workflow

In addition, Applied BioProbes' iQuant™ DNA Quantitation Kits provide higher sensitivity than absorbance-based quantitation methods, such as NanoDrop (Figure 2).

                             
Figure 2. Improved sensitivity with iQuant™ DNA Quantitation Kits

Further, iQuant™ DNA Quantitation Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Figure 3).

Figure 3. Selectivity for dsDNA using iQuant™ HS dsDNA Quantitation Kit in the presence of an equal mass of RNA.

iQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with NanoDrop, especially at relatively low DNA concentrations (Figure 4).

Figure 4. Comparison of accuracy of DNA quantitation between the iQuant™ HS dsDNA Quantitation Kit and NanoDrop.

The iQuant™ DNA kits for DNA quantitation are simple to use. Just mix your DNA sample with iQuant™ dye solution, and perform the assays in either a multi-well plate reader, or in single tubes (Figure 5). The iQuant™ kits are compatible with the Qubit® fluorometer from Life Technologies and the Quantus™ fluorometer from Promega.

Figure 5. Assay workflow for iQuant™ DNA Quantitation Kits