聯(lián)系電話
上海起發(fā)實(shí)驗(yàn)試劑有限公司
中級(jí)會(huì)員·16年
- 聯(lián)系人：
- 楊經(jīng)理
- 電話：
- 021-50724187 021-50724961
- 手機(jī)：
- 15921799099
- 傳真：
- 021-50724961
- 地址：
- 上海浦東川沙鎮(zhèn)川沙路6619號(hào)上海起發(fā)實(shí)驗(yàn)試劑有限公司
- 個(gè)性化：
- www.qfbio.com
- 網(wǎng)址：
- www.qfbio.com
掃一掃訪問手機(jī)商鋪
PAP (Pyrophosphorolysis-activated polymerization)/ KlenTaq-S
PAP is a unique method for DNA amplification (Liu and Sommer: BioTechniques, 29 [2000], 1072-1080; Nucleic Acids Res, 30 [2002], 598-604), where pyrophosphorolysis and polymerization are serially coupled by DNA polymerase using 3’ blocked primer. Pyrophosphorolysis is the reverse reaction of DNA polymerization where the blocked nucleotide (generally dideoxy) at the 3’ end of the primer template duplex is removed by the enzyme in presence of inorganic pyrophosphate. After the removal of the blocked nucleotide by pyrophosphorolysis, the activated primer can then be extended by DNA polymerization.
Because of the high specificity (1 / 3x1011) of PAP, this process is becoming a powerful tool for detecting rare mutations, large heterozygous deletions, gene duplications etc in the presence of a large excess of wild type allele (Liu et al : Biotechniques, 40[2006], 661-668). In theory PAP can detect a single base mutation in 3x1011 copies of wild type allele.
PAP can be used as an alternative or to substantiate the Real Time PCR (Boon et al : Prenat Diagn 27[2007], 932-937)
KlenTaq-S and PAP reaction.
KlenTaq-S has been found to be a suitable thermostable Taq DNA polymerase by many research groups in PAP reactions.
KlenTaq-S is expressed from 5’ deletion and F667Y (Tabor and Richardson) mutation of the gene, encoding Thermus aquaticus DNA polymerase. Repeated exposure to 980C in the PR1 reaction buffer the enzyme does not show any noticeable loss of activity.
Concentration of KlenTaq-S : 10 U/µl
Suggested Reaction Conditions for PAP (in 25 µl) :
50 mM Tris-HCl, 7.8, 16 mM Ammonium sulfate, 3.5 mM Magnesium chloride, 25 µM each four dNTPs, 0.1 µM each primer, 5-200 ng template, 90 µM inorganic pyrophosphate (Na4PPi), 2% dimethylsulfoxide (DMSO), 2.5 U of KlenTaq-S.
Cycling Conditions :
950C for 1 min (denaturation), then
940C for 15 sec
600C for 30 sec
640C for 30 sec
680C for 1 min
720C for 1-2 min total of 25-45 cycles
Buffer supplied (10X PR1):
500 mM Tris-HCl, pH 7.8, 160 mM (NH4)2SO4, 35 mM MgCl2, 1.5 mg/ml BSA
Ordering Information :
Order No. : KTS-01
Quantity : 100 µl or 1000 U/vial
| 貨號(hào) | 品名 | 規(guī)格 | 品牌 |
| KTS-01 | PAP (Pyrophosphorolysis-activated polymerization) | 1000 U | KlenTaq-S |
我們公司zui大優(yōu)勢是強(qiáng)大的采購，
1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，
2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，
3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免
4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證100%產(chǎn)品都是,因?yàn)橐馕吨鴽]有服務(wù).
5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大 交大 復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥 ，fisher等500多家公司
6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。
