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CAS号9001-12-1胶原酶Ⅰ
货号：C8140
规格：100mg/支
外观（性状） : 棕色或棕褐色结晶性粉末
储存条件 : -20℃
纯度 : 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid
英文名称 : CollagenaseⅠ
单位 : 支
别名 : 梭菌肽酶 A
| 商品货号： | 商品品牌： | 规格 | 基本售价： | 选择规格 |
|---|---|---|---|---|
| C8140-100mg | Solarbio | 100mg | 440.00元 |
说明：胶原酶Ⅰ用于上皮、肺，脂肪和肾上腺组织细胞的分离
胶原蛋白酶 来源于溶组织梭菌
Collagenase from Clostridium histolyticum
for general use, Type I, 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.
Caution
As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains * activity over 7 hours when held on ice.
Preparation Note
Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953).
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximay 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.
Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Cell Dissociation, Cell Dissociation and Cell Lysis,
Collagenases, Core Bioreagents, Crude Collagenase For General Use, Enzyme Class Index, Enzymes, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Research Essentials, 生化试剂, 酶、抑制剂和底物
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form essentially salt-free, lyophilized powder
solubility TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble1-2 mg/mL at 37 °C
storage temp. −20°C
CAS号9001-12-1胶原酶Ⅰ订购说明：
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CAS号9001-12-1胶原酶Ⅰ运输说明：
1、极低温产品：极低温产品运输过程中加装干冰运输。用干冰把产品包裹起来，再用泡沫盒密封，胶带层层粘住泡沫盒，放入索莱宝的箱子，然后交付给合作快递，安全快速高效放心的送客户的手中，保证产品的性质稳定如一，为您的实验保驾护航。
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