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重組人內(nèi)分泌腺血管內(nèi)皮生長因子(rHuEG-VEGF)

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CYT-338重組人內(nèi)分泌腺血管內(nèi)皮生長因子(rHuEG-VEGF) 2μg/10μg/1mg Prospec

 

 

Background:
 Human endocrine gland derived vascular endothelial growth factor (EG-VEGF) is selectively expressed in steroidogenic glands and promotes growth of endocrine gland endothelium. The identification of tissue-selective angiogenic factors raises the possibility that other secreted molecules in this class exist. Consistent with such an expression pattern, the human EG-VEGF gene promoter has a potential binding site for steroidogenic factor (SF)-1, a pivotal element for steroidogenic-specific transcription. In the human ovary, the expression of EG-VEGF is temporally and spatially complementary to the expression of VEGF-A, both in the follicular and in the luteal phase, suggesting complementary and coordinated roles of these molecules in ovarian angiogenesis. Also, EG-VEGF expression correlates with vascularity in the polycystic ovary syndrome, a leading cause of infertility.

 
Description :
 Recombinant Human EG-VEGF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9605 Dalton.

Recombinant EG-VEGF is purified by proprietary techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 Recombinant VEGF was lyophilized from a concentrated (1mg/ml) solution with no additives.

 
Solubility:
 It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuVEGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Ile-Thr-Gly.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of EG-VEGF.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of EG-VEGF as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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