MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)。

Rhodamine 123

CAS No. : 62669-70-9

產(chǎn)品活性：Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

研究領(lǐng)域：Others

作用靶點(diǎn)：Fluorescent Dye

In Vitro: 1.Preparation of Rhodamine 123 working solution
1.1Preparation of the stock solution
Dissolve 1 mg Rhodamine 123 in 525 μL DMSO to obtain 5 mM of stock solution.
1.2Preparation of Rhodamine 123 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
Note: Please adjust the concentration of Rhodamine 123 working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.

相關(guān)產(chǎn)品：MeOSuc-Ala-Ala-Pro-Val-pNA  |  BDP 558/568 amine  |  AC-green  |  Naphthalene-1,2-dicarbaldehyde  |  Rhodamine 800  |  Vari Fluor 532 SE  |  Acridine Orange hydrochloride  |  N-(7-Nitrobenzofurazan-4-yl)phallacidin  |  Biotin-11-UTP  |  BODIPY FL SSE  |  1,2-Diamino-3,4-ethylenedioxybenzene  |  Sulfo-Cyanine5.5 NHS ester tripotassium  |  BODIPY 505/515  |  3-Acetylumbelliferyl β-D-Glucopyranoside  |  Ac-DNLD-AMC  |  Mucicarmine  |  7-Aminocoumarin  |  NEP  |  Sulfo-cyanine3 azide sodium  |  Dihydrorhodamine 123  |  N-Acryloyl-1-pyrenebutylamine  |  TSPP tetrasodium  |  Dabcyl acid  |  7-Diethylamino-4-methylcoumarin  |  9-(2-Carboxy-2-cyanovinyl)julolidine  |  RT-NH2  |  Coumberol  |  Carmoisine  |  4-Methylumbelliferyl-N-acetyl-α-D-glucosaminide  |  MitoMark Red I

熱門產(chǎn)品線：重組蛋白  |  化合物庫  |  天然產(chǎn)物  |  熒光染料  |  PROTAC  |  同位素標(biāo)記物  |  寡核苷酸  |  抗體  |  點(diǎn)擊化學(xué)

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Fluorescent Dye  |  PROTAC  |  Isotope-Labeled Compounds  |  Oligonucleotides  |  Antibodies  |  Click Chemistry

品牌介紹:
•   MCE (MedChemExpress) 擁有200 多種*僅有化合物庫，我們致力于為*科研客戶提供前沿的高品質(zhì)小分子活性化合物；
•   50,000 多種高選擇性抑制劑、激動(dòng)劑涉及各熱門信號通路及疾病領(lǐng)；
•   產(chǎn)品種類涵蓋各種重組蛋白，多肽，常用試劑盒 ，更有 PROTAC、ADC 等特色產(chǎn)品，廣泛應(yīng)用于新藥研發(fā)、生命科學(xué)等科研項(xiàng)目；
•   提供虛擬篩選，離子通道篩選，代謝組學(xué)分析檢測分析，藥物篩選等專業(yè)技術(shù)服務(wù)；
•   設(shè)有專業(yè)的實(shí)驗(yàn)中心和嚴(yán)格的質(zhì)控、驗(yàn)證體系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各項(xiàng)質(zhì)檢報(bào)告，確保產(chǎn)品的高純度、高品質(zhì)；
•   產(chǎn)品的生物活性多經(jīng)各國客戶實(shí)驗(yàn)驗(yàn)證；
•   Nature, Cell, Science 等多種頂級期刊及制藥 Patent 收錄了MCE客戶的科研成果；
•   專業(yè)團(tuán)隊(duì)跟蹤新的制藥及生命科學(xué)研究進(jìn)展，為您提供*新的活性化合物；
•   與世界各大制藥公司及*科研機(jī)構(gòu)建立了長期的合作。