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IMDM

Product Overview:

IMDM (Iscove's Modified Dulbecco's Medium) is utilized not only for more specialized cell types but also as the basis for some of the more unique serum-free media formulations. The original formulation designed by Iscove and associates, demonstrated that erythrocytes and macrophage precursor cells (e.g. primary Haemopoietic Cells) could be cultured in a totally defined serum-free medium when supplemented with albumin, lecithin, selenium and transferrin. is a modification of DMEM (Dulbecco's Modified Eagle's Medium) containing selenium, additional AA's, vitamins and other components. is a highly enriched synthetic media well-suited for rapidly proliferating and high-density cell cultures in a 5% CO2 atmosphere.

medium reportedly supports Murine B Lymphocytes, Haemopoietic tissues from Bone Marrow, B-cells stimulated with Lipopolysaccharide, and also T-Lymphocytes and a wide variety of Hybrid cells. When utilized in conjunction with serum, it supports a broad spectrum of Mammalian cell types including but not exclusive to bone marrow, hybridomas and lymphocytes.

This is modified as follows:

With

• L-Glutamine

• Phenol Red

• Sodium Bicarbonate

• HEPES

Without:

• Beta Mercaptoethanol

• Alpha-Thioglycerol