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目錄:愛必信(上海)生物科技有限公司>>抗體>>內(nèi)參抗體>> abs100006Beta-Actin Antibody(50ul)

Beta-Actin Antibody(50ul)
  • Beta-Actin Antibody(50ul)
參考價 1100
訂貨量 ≥1
具體成交價以合同協(xié)議為準(zhǔn)
1100
≥1
具體成交價以合同協(xié)議為準(zhǔn)
  • 品牌 absin
  • 型號 abs100006
  • 廠商性質(zhì) 生產(chǎn)商
  • 所在地 上海市
屬性

供貨周期:兩周

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更新時間:2025-03-25 08:33:47瀏覽次數(shù):2066評價

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生化試劑(60萬種)、抗體(10萬種)、細胞因子、二抗、ECL發(fā)光液、蛋白marker、激動劑、抑制劑、凋亡試劑盒、BSA、動植物提取物、蛋白酶K...
概述
描述Purified Mouse Monoclonal Antibody (Mab)
別名Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
克隆類別Monoclonal
克隆號8H10D10
反應(yīng)種屬 H, M, R
來源宿主Mouse
應(yīng)用WB, IHC-P
形態(tài)Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediay, followed by dialysis against PBS.
使用方法WB~~1:1000
IHC-P~~1:10~50
性能
生產(chǎn)說明ACTB recombinant protein is used to produce this monoclonal antibody.
抗原來源HUMAN
背景This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
保存方法Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
研究領(lǐng)域Crown Antibodies;Cancer;Signal Transduction;Loading Control
分子量41.7 kDa
參考文獻
Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.

實驗結(jié)果圖

Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right),  using Beta-actin Antibody(Cat.  #abs100006).  abs100006 was diluted at 1:1000 at each lane.  A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right), using Beta-actin Antibody(Cat. #abs100006). abs100006 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(Cat.  #abs100006). abs100006 was diluted at 1:1000 at each lane.  A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(Cat. #abs100006). abs100006 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

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