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Cellartis定型内胚层干细胞

产品英文名称：Cellartis Definitive Endoderm Cells (from ChiPSC18)

产品中文名称：Cellartis定型内胚层干细胞

适合用于进一步诱导分化成为肝脏细胞类型或是胰脏细胞类型。

Cellartis定型内胚层干细胞（DE cell）是从hiPS细胞株ChiPSC18经体外诱导分化得来。该产品中，大于90%的细胞显示表达SOX17蛋白。该产品为低温冷冻保存的细胞悬浮形式。本产品适合用于进一步诱导分化成为肝脏细胞类型或是胰脏细胞类型。特别地，Cellartis的肝脏细胞诱导分化试剂盒（Cellartis Hepatocyte Differentiation Kit，Cat. No. Y30050）适合用于DE向肝脏细胞类型的诱导分化工作。

在产生Cellartis定型内胚层细胞（来自ChiPSC18）的分化过程中，通过RT-qPCR测量的mRNA表达谱显示内胚层标志物SOX17，FOXA2，FZD5，CXCR4，HHEX和SOX7的预期时间表达; 中胚层标记Brachyury; 和多能标记OCT4。

Cellartis Definitive Endoderm Cells (from ChiPSC18) are human definitive endoderm (DE) cells derived from the human induced pluripotent stem (hiPS) cell line ChiPSC18. The hiPS cells have been differentiated into definitive endoderm cells in vitro to generate a population of >90% SOX17-protein-expressing cells; they are then dissociated into a cell suspension and cryopreserved. These DE cells are suitable for further differentiation into hepatocytes or pancreatic endoderm cells. To differentiate the DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050).

During the differentiation process that creates Cellartis Definitive Endoderm Cells (from ChiPSC18), the mRNA expression profile—as measured by RT-qPCR—shows the expected temporal expression of endodermal markers SOX17, FOXA2, FZD5, CXCR4, HHEX, and SOX7; mesoendodermal marker Brachyury; and pluripotency marker OCT4. See the Images & Data tab, below.

产品特点:

具有> 90％SOX17阳性细胞的定形内胚层细胞

可用于下一步分化内胚层起源的下级分化细胞，如肝细胞或胰腺内胚层

Definitive endoderm cells with >90% SOX17-positive cells

Can be used for generating further-specified cells of endodermal origin, such as hepatocytes or pancreatic endoderm

组份：

Cellartis Definitive Endoderm Cells (from ChiPSC18) (Cat. No. Y10040)

保存：Cellartis Definitive Endoderm Cells (from ChiPSC18) should be stored at ≤ –150°C. Under

recommended storage conditions, the cells can be stored for up to one year from the date of receipt.

Culture thawed Cellartis Definitive Endoderm Cells (from ChiPSC18) at 37°C ± 1°C, 5% CO2 and>90% humidity.

产品性能：

Immunocytochemistry of Cellartis Definitive Endoderm Cells (from ChiPSC18). Cells were thawed, cultured for two days, fixed, and stained for the pluripotency marker OCT4, the definitive endoderm marker SOX17, and the nuclear marker DAPI. Results show that more than 90% of the cell population are definitive endoderm cells and have lost pluripotency.

相关产品：

Cellartis DEF-CS™ 500 COAT-1 (Takara Bio, Cat. No. Y30012)

iMatrix-511 (Takara Bio, Cat. No. T303)

iMatrix-511 (Takara Bio, Cat. No. T304)

Cellartis Hepatocyte Differentiation Kit (Takara Bio, Cat. No. Y30050)

肝细胞培养完整解决方案

人iPS诱导分化成肝细胞全过程的完整试剂盒：Y30055（新产品）

人iPS来源肝细胞株及*培养基Y10133/Y10134/Y10135（新产品）

人原代肝脏细胞培养基 Y20020（新产品）

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华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司主营细胞治疗和精准医疗等产品，2018年与TAKARA继续合作，授权代理（上海）其旗下Cellartis干细胞研究制品和Rubicon单细胞文库构建试剂。Cellartis公司位于瑞典，拥有iPS细胞等干细胞分化为肝细胞及脏器细胞的分化诱导技术和ES细胞，ips细胞，分化细胞等干细胞相关产品。

Cellartis-Y10040 Human Stem Cell Derived Definitive Endoderm Cells 及更多细胞培养基产品咨询电话 手机1500 1904 520 红荣微再-客服：  经销商专员：