Recombinant Transmembrane Protein 27 (TMEM27)  

跨膜蛋白27(TMEM27)重組蛋白

[ PROPERTIES ]

Residues: Cys17~Asp137,with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Membrane; Single-passtype I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.01%sarcosyl and preservative.

Predicted isoelectric point: 7.9

Predicted Molecular Mass: 15.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8°Cfor one month.

             Aliquot and store at -80°Cfor 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-CQPG AENAFKVRLS IRTALGDKAY AWDTNEEYLF KAMVAFSMRKVPNREATEIS HVLLCNVTQR VSFWFVVTDP SKNHTLPAVE VQSAIRMNKNRINNAFFLNDQTLEFLKIPS TLAPPMD

[  IDENTIFICATION  ]

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