FcεRI Effector Reporter Cell
| I. Background | |
IgE由两条重链（ε链）和两条轻链组成，Fc段含高亲和力受体（FcεRI）结合位点，半衰期仅2-3天（IgG为21天）。作为免疫系统的“哨兵"，IgE既可结合寄生虫表面抗原，激活嗜酸性粒细胞释放毒性颗粒（如主要碱性蛋白），通过ADCC（抗体依赖细胞介导的细胞毒性）清除寄生虫（如血吸虫、钩虫）；也能因过度反应引发致命过敏；IgE还可靶向肿瘤抗原（如HER2），激活巨噬细胞ADCC效应；并且最近有研究指出，脑膜肥大细胞通过IgE-MRGPRX2可加剧阿尔茨海默病病理。 | |
| II. Description | |
FcεRI Effector Reporter Cell 药靶模型很好的模拟了 FcεRI的信号转导过程，原理见下图所示。
Figure 1. FcεRI Effector Reporter Cell细胞模型原理图 | |
| III. Introduction | |
| Expressed gene: | FcεRI |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+ 1 μg/ml Puromycin+ 10 μg/ml Blasticidin + 800 μg/ml Hygromycin B+ 1000 μg/ml G418 |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data
Figure 2. Recombinant FcεRI Effector Reporter Cell stably expressing FcεRIA.
Figure 3. Dose Response of Recombinant Human IgE in FcεRI Effector Reporter Cell(C4).
Figure 4. Inhibition of Human IgE-induced Reporter Activity by Human IgE Neutralization Ab in FcεRI Effector Reporter Cell(C4). | |
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