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NUCLEOSIL® NH2 / NH2 RP 液相色譜柱
Nucleosil 高性能硅膠，特殊技術鍵合，使NH2鍵合相在水相介質(zhì)中穩(wěn)定，低流失，保證柱子長壽命※ 鍵合相采用非端基封尾，具有更廣泛的選擇性※ 有100, 120 and 300 Å，三種孔徑；有3um, 5um, 7um, 10um 四種粒度

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Nucleosil NH2 氨基柱

※ Nucleosil 高性能硅膠，特殊技術鍵合，使NH2鍵合相在水相介質(zhì)中穩(wěn)定，低流失，保證柱子長壽命※ 鍵合相采用非端基封尾，具有更廣泛的選擇性※ 有100, 120 and 300 Å，三種孔徑；有3um, 5um, 7um, 10um 四種粒度

※ pH stability at 20 °C: 2–8, carbon content 3.5 %※      可以使用多種流動相體系，分析不同性質(zhì)樣品：
    * 正相流動相（正乙烷，二氯乙烯，異丙醇），分析苯胺取代物，酯類和有機氯農(nóng)藥
    * 水和有機物作為正相流動相，可以分析極性樣品，如：糖類、植物甙類
    * 反相中添加緩沖鹽，作為弱離子交換體系可以分析有機酸類，陰離子化合物

NUCLEOSIL® NH2 / NH2 RP
Amino modified silica phases for HPLC · USP L8

Features

Polar aminopropyl modified silica for reversed phase and normal phase chromatography:
Normal phase chromatography with hexane, dichloromethane or 2-propanol as phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
Reversed phase chromatography of polar compounds like carbohydrates in aqueous-organic eluent systems
Anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile)

Available pore sizes 100, 120 and 300 Å, available particle sizes 3 µm, 5 µm, 7 µm and 10 µm,
pH stability at 20 °C: 2–8, carbon content 3.5 %

Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions

Corresponding NUCLEODUR® phase see NUCLEODUR® NH2 / NH2-RP

For applications click here.

產(chǎn)品應用:

雙糖檢測

色譜柱：Nucleosil 100-5 NH2, 250×4 mm

流動相：乙腈：水=75:25

流速：1毫升/分鐘

樣品：1. 甲基葡萄糖苷 2. 果糖 3. 葡萄糖 4. 蔗糖 5. 乳糖 6. 麥芽糖

HPLC檢測 Curcumin （姜黃素）及相關組份

色譜柱：Nucleosil 100-5 NH2, 250×4.6 mm ID

流動相：96% 乙醇

流速：1.2毫升/分鐘

檢測器：fluorescence, excitation 420nm, emission 470nm. The curcumino ids can also be detected by UV-VIS spectroscopy at 254 or 420nm.

樣品：1. Bisde methoxycurcumin 2. Demethoxycurcumin 3.Curcumin

發(fā)酵液中多糖的檢測

色譜柱：Nucleosil 100-5 NH2, 250×4 mm

流動相：乙腈：水=65:35

流速：2毫升/分鐘

溫度：80℃
樣品：1. 葡萄糖 2. 纖維素二糖 3-8.不同聚合度葡聚糖

訂貨信息:

EC standard columns for analytical HPLC
Ordering information
Eluent in column (except for NUCLEOSIL® 100-5 NH2 RP) is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first.

ID	Particle size	Pore size		Length 250 mm		EC guard columns*	CC guard columns**
NUCLEOSIL® 100-3 NH2 (eluent in column n-heptane)
4.6 mm	3 µm	100 Å		720275.46		721933.30	721122.40
NUCLEOSIL® 100-5 NH2 RP (eluent in column acetonitrile – water)
4.6 mm	5 µm	100 Å		720095.46RP		721155.30	721605.40RP
NUCLEOSIL® 100-5 NH2 (eluent in column n-heptane)
4.6 mm	5 µm	100 Å		720095.46		721020.30	721605.40
NUCLEOSIL® 100-10 NH2 (eluent in column n-heptane)
4.6 mm	10 µm	100 Å		720025.46
Columns/pack ->				1		3	3
* EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966). CC 8/4 guard column cartridges are used for EC columns of 4.6 mm ID with guard column adapter EC (REF 721359)**.
Custom-packed EC columns with other dimensions are available on request.
NUCLEOSIL® NH2 bulk packingsOrdering information
Phase		Carbon content	Pore size	Particle size	Pack of 10 g	Pack of 100 g
NUCLEOSIL 100-5 NH2		3.5 %	100 Å	5 µm	712200.10	712200.100
NUCLEOSIL 100-10 NH2		3.5 %	100 Å	10 µm	712210.10	712210.100
NUCLEOSIL 120-7 NH2		~ 2 %	120 Å	7 µm	712610.10	712610.100
NUCLEOSIL 300-7 NH2		~ 2 %	300 Å	7 µm	712919.10	712919.100

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