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技術(shù)文獻(xiàn):小鼠CRH,ACTH,CORT ELISA試劑盒引用文獻(xiàn)

【文獻(xiàn)標(biāo)題】Reduced growth capacity of preimplantation mouse embryos in chronic unpredictable stress model

【作者】Xiaoli Zhao，Ruihong Ma，Xiaoyu Zhang，et.al

【作者單位】天津中醫(yī)藥大學(xué)第YI附屬醫(yī)院（ First Teaching Hospital of Tianjin University of Traditional Chinese Medicine）

【文獻(xiàn)中引用產(chǎn)品】

小鼠促腎上腺皮質(zhì)激素釋放激素（CRH）ELISA試劑盒

小鼠促腎上腺皮質(zhì)激素（ACTH）ELISA試劑盒

小鼠皮質(zhì)酮/腎上腺酮（CORT）ELISA試劑盒

【關(guān)鍵詞】chronic unpredictable stress model, differential gene expression, hypothalamus‐pituitary‐adrenal axis, preimplantation embryos, RNA sequencing

【DOI】DOI: 10.1002/mrd.23439

【影響因子(IF)】2.82

【出版期刊】《Molecular Reproduction and Development》

【產(chǎn)品原文引用】

4.4 | Measurement of serum HPA axis‐associated

hormones

Blood was collected from 20 mice in each group by retro‐orbital bleeding. For serum preparation, the blood samples were left undisturbed at room temperature for 30 min, and then subject to centrifugation at 1000g for 15 min at 4°C. The supernatants were transferred into new tubes and stored at ?80°C until analysis. Serum levels of CRH, ACTH, and CORT were measured by ELISA according to manufacturer's manual (H‐20509, H‐20423, H‐20381, respectively; Shanghai Hengyuan Biotechnology Inc.).

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